CAJ | 학술논문

目的探讨孕期吸入异氟醚对子代大鼠海马神经元突触可塑性的影响.方法孕14 d的SD大鼠10只,体重220～250 g,采用随机数字表法,将孕鼠随机分为对照组和异氟醚组,每组5只.对照组大鼠每天单纯机械通气2 h,异氟醚组大鼠每天吸入1.3%异氟醚2 h,至大鼠分娩.子代大鼠出生后4周,采用Morris水迷宫实验测定认知功能,测定结束后处死子代大鼠取脑,分离海马,采用透射电镜观察海马CA1区神经元突触的超微结构,计数海马神经元突触数量,测定突触后致密物质厚度.结果与对照组相比,异氟醚组子代大鼠逃避潜伏期延长,穿越平台次数减少,海马神经元突触数量减少,突触后致密物质厚度降低(P<0.05).结论孕期吸入异氟醚可通过抑制子代大鼠海马神经元突触的可塑性而降低其认知功能.
목적 탐토잉기흡입이불미대자대대서해마신경원돌촉가소성적영향.방법 잉14 d적SD대서10지,체중220～250 g,채용수궤수자표법,장잉서수궤분위대조조화이불미조,매조5지.대조조대서매천단순궤계통기2 h,이불미조대서매천흡입1.3%이불미2 h,지대서분면.자대대서출생후4주,채용Morris수미궁실험측정인지공능,측정결속후처사자대대서취뇌,분리해마,채용투사전경관찰해마CA1구신경원돌촉적초미결구,계수해마신경원돌촉수량,측정돌촉후치밀물질후도.결과 여대조조상비,이불미조자대대서도피잠복기연장,천월평태차수감소,해마신경원돌촉수량감소,돌촉후치밀물질후도강저(P<0.05).결론 잉기흡입이불미가통과억제자대대서해마신경원돌촉적가소성이강저기인지공능.
Objective To investigate the effect of isoflurane inhalation during gestation period on plasticity of hippo-campal synapses in offspring rats. Methods Ten healthy pregnant SD rats at 14 day gestation were randomly divided into 2 gorups ( n = 5 each): control group (group C) and isoflurane group (group I). The rats in group C were mechanically ventilated with O2 while in group I the rats inhaled 1.3% isoflurane in O2 for 2 h a day until labor. Four weeks after birth 4 offspring rats from each pregnant rats (2 male, 2 female) were tested for learning and memory abilities using Morris water maze. Then the offspring rats were sacrificed and hippocampi isolated. The synaptic structure of hippocampal CA1 area was examined by trans-electron microscopy. Results Morris water maze test showed that the escape latency was significantly shorter and the number of times of spanning flat roof greater in group C than in group I. The structure of hippocampus was intact in group C but incomplete in group I. Meanwhile the thickness of synaptic density was significantly decreased in group I. Conclusion Isoflurane anesthesia of pregnant rats may induce learning and memory disabilities in offspring rats by inhibiting the plasticity of synaptic structure in hippocampus.