CAJ | 학술논문

目的寻找可能的骨肉瘤血清蛋白标志物,探讨溶酶体相关基因在骨肉瘤中的表达和功能.方法分别利用基因芯片和激光解析离子化飞行时间质谱(SELDI-TOF-MS)技术筛选3个骨肉瘤细胞株和27例骨肉瘤患者血清中差异表达的基因和血清蛋白质峰.采用Link-test统计学方法筛选得到骨肉瘤血清标志蛋白,采用MATLAB软件对653个差异表达的标志蛋白进行功能聚类分析,并对溶酶体相关基因进行KEGG分析.结果本实验在骨肉瘤细胞株中共筛选出653个候选骨肉瘤标志基因,在骨肉瘤患者血清中共筛选到9个差异表达的蛋白质谱峰.通过Link-test统计分析,共筛选到15个差异表达的骨肉瘤血清标志蛋白.骨肉瘤相关基因SCARB2、GNS和DNASE2等在溶酶体信号通路中显著富集.结论溶酶体相关基因表达失调和溶酶体功能紊乱是骨肉瘤形成的原因之一,可以作为骨肉瘤的诊断标志物.溶酶体可以作为骨肉瘤治疗的靶细胞器.
목적 심조가능적골육류혈청단백표지물,탐토용매체상관기인재골육류중적표체화공능.방법 분별이용기인심편화격광해석리자화비행시간질보(SELDI-TOF-MS)기술사선3개골육류세포주화27례골육류환자혈청중차이표체적기인화혈청단백질봉.채용Link-test통계학방법 사선득도골육류혈청표지단백,채용MATLAB연건대653개차이표체적표지단백진행공능취류분석,병대용매체상관기인진행KEGG분석.결과 본실험재골육류세포주중공사선출653개후선골육류표지기인,재골육류환자혈청중공사선도9개차이표체적단백질보봉.통과Link-test통계분석,공사선도15개차이표체적골육류혈청표지단백.골육류상관기인SCARB2、GNS화DNASE2등재용매체신호통로중현저부집.결론 용매체상관기인표체실조화용매체공능문란시골육류형성적원인지일,가이작위골육류적진단표지물.용매체가이작위골육류치료적파세포기.
Objective To discover potential marker proteins of osteosarcoma in serum, and explore the expression and function of lysosome related genes in osteosarcoma. Methods Gene chip and SELDI-TOF-MS were respectively used to screen genes and protein peaks in 3 cell lines and 27 patients with osteosarcoma. Link-test was used to discover 653 potential markers of osteosarcoma in serum. Enrichment analysis of GO terms and KEGG pathways was performed by MATLAB. Results 653 candidate osteosarcoma maker genes were found in osteosarcoma cells, while 9 protein peaks were differentially expressed in serum of patients with osteosarcoma. By using link-test, 15 differentially expressed osteosarcoma serum makers were screened. Osteosarcoma related genes were significantly enriched in lysosome pathway (SCARB2, GNS and DNASE2). Conclusion Un-regulation of lysosome related genes and dysfunction of lysosome would be one reason of osteosarcoma tumorigenesis, and may serve as a diagnositic marker. Lysosome could be used as an important therapy target of osteosarcoma.