中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2012年
10期
748-752
,共5页
常秀娟%王春平%曲建慧%陆荫英%陈艳%白文林%高旭东%郝莉燕%许桂林%王鋐%杨永平
常秀娟%王春平%麯建慧%陸蔭英%陳豔%白文林%高旭東%郝莉燕%許桂林%王鋐%楊永平
상수연%왕춘평%곡건혜%륙음영%진염%백문림%고욱동%학리연%허계림%왕횡%양영평
癌,肝细胞%结肠癌转移相关基因子1%复发%预后
癌,肝細胞%結腸癌轉移相關基因子1%複髮%預後
암,간세포%결장암전이상관기인자1%복발%예후
Carcinoma,hepatocellular%Metastasis-associated in colon cancer 1%Recurrence%Prognosis
目的 探讨结肠癌转移相关基因1(MACC1) mRNA在肝细胞癌中的表达及其临床意义.方法 通过实时荧光定量PCR和免疫组化分别检测MACC1在354例肝癌组织中和10例正常肝组织的表达及其分布.采用单因素分析和Cox多因素回归分析MACC1 mRNA表达的影响因素.结果 MACC1 mRNA在早期、中期和进展期肝癌组织中的中位相对表达量分别为0.001 76、0.002 49和0.008 35,与正常对照组0.000 59比较,差异均有统计学意义(均P＜0.01).MACC1低表达组患者年龄、分期、瘤栓、分化程度与高表达组比较,差异均有统计学意义(均P＜0.05).肝癌组织中MACC1表达的阳性率为42.4％(150/354),MACC1在正常肝细胞质呈阴性或弱阳性表达,部分患者肿瘤细胞核染色呈阳性.MACC1 mRNA高表达者中位无瘤生存时间(34.0个月)和总生存时间(40.0个月)均低于低表达者(均为48.0个月,均P＜0.01).Cox多因素;归分析结果显示,Child-Pugh分级、MACC1 mRNA高表达是影响患者生存期和无瘤生存时间的独立影响因素.结论 肝癌组织中MACC1 mRNA高表达与疾病进展和预后有关,可能成为肝癌术后复发、转移的潜在预测因子.
目的 探討結腸癌轉移相關基因1(MACC1) mRNA在肝細胞癌中的錶達及其臨床意義.方法 通過實時熒光定量PCR和免疫組化分彆檢測MACC1在354例肝癌組織中和10例正常肝組織的錶達及其分佈.採用單因素分析和Cox多因素迴歸分析MACC1 mRNA錶達的影響因素.結果 MACC1 mRNA在早期、中期和進展期肝癌組織中的中位相對錶達量分彆為0.001 76、0.002 49和0.008 35,與正常對照組0.000 59比較,差異均有統計學意義(均P＜0.01).MACC1低錶達組患者年齡、分期、瘤栓、分化程度與高錶達組比較,差異均有統計學意義(均P＜0.05).肝癌組織中MACC1錶達的暘性率為42.4％(150/354),MACC1在正常肝細胞質呈陰性或弱暘性錶達,部分患者腫瘤細胞覈染色呈暘性.MACC1 mRNA高錶達者中位無瘤生存時間(34.0箇月)和總生存時間(40.0箇月)均低于低錶達者(均為48.0箇月,均P＜0.01).Cox多因素;歸分析結果顯示,Child-Pugh分級、MACC1 mRNA高錶達是影響患者生存期和無瘤生存時間的獨立影響因素.結論 肝癌組織中MACC1 mRNA高錶達與疾病進展和預後有關,可能成為肝癌術後複髮、轉移的潛在預測因子.
목적 탐토결장암전이상관기인1(MACC1) mRNA재간세포암중적표체급기림상의의.방법 통과실시형광정량PCR화면역조화분별검측MACC1재354례간암조직중화10례정상간조직적표체급기분포.채용단인소분석화Cox다인소회귀분석MACC1 mRNA표체적영향인소.결과 MACC1 mRNA재조기、중기화진전기간암조직중적중위상대표체량분별위0.001 76、0.002 49화0.008 35,여정상대조조0.000 59비교,차이균유통계학의의(균P＜0.01).MACC1저표체조환자년령、분기、류전、분화정도여고표체조비교,차이균유통계학의의(균P＜0.05).간암조직중MACC1표체적양성솔위42.4％(150/354),MACC1재정상간세포질정음성혹약양성표체,부분환자종류세포핵염색정양성.MACC1 mRNA고표체자중위무류생존시간(34.0개월)화총생존시간(40.0개월)균저우저표체자(균위48.0개월,균P＜0.01).Cox다인소;귀분석결과현시,Child-Pugh분급、MACC1 mRNA고표체시영향환자생존기화무류생존시간적독립영향인소.결론 간암조직중MACC1 mRNA고표체여질병진전화예후유관,가능성위간암술후복발、전이적잠재예측인자.
Objective To clarify the expression and clinical significance of metastasis-associated in colon cancer 1 (MACC1) mRNA in hepatocellular carcinoma (HCC).Methods The expression and distribution of MACC1 were assessed by quantitative real-time polymerase chain reaction (RT-PCR) and immunohistochemical staining (IHC) in a cohort of hepatitis B virus-related HCC,including 138 in early (A),96 in intermediate (B) and 120 in advanced stages (C).The association of MACC1 mRNA with disease progression and outcomes was analyzed by univariate and multivariate Cox analysis.Results The intratumoral expressions of MACC1 mRNA in HCC stage Ⅰ (0.001 76,range:0.000 54-0.002 47),stage Ⅱ (0.002 49,range:0.000 55-0.006 78) and stage Ⅲ (0.008 35,range:0.006 86-0.009 88) were about 3-,4-and 14-fold higher than that in the normal liver tissue (0.000 59,range:0.000 57-0.000 60),respectively.Intratumoral expression of MACC1 mRNA increased with disease progression from stage Ⅰ to stage Ⅲ.HCC clinical staging classification,age,portal vein invasion and tumor differentiation were significantly associated with intratumoral high expression of MACC1 mRNA (All P ＜ 0.05).Immunohistochemical staining showed that there was an increased MACC1 expression in cytoplasm of HCC cells and positive nuclear staining in some cases.Increased MACC1 mRNA expression could predict poor outcome and recurrence in stage A and B HCC postoperatively.The median tumor-free survival and total survival of patients with high MACC1 mRNA expression were 34.0 and 40 months,respectively,significantly lower than that in those with low expression (48.0 and 48.0 months) (all P ＜ 0.01).Cox analysis showed that Child-Pugh grading and high expression of MACC1 mRNA were independent predictive factors,and high expression of MACC1 was an independent predictive factor affecting the tumor-free survival.Conclusions MACC1 mRNA up-regulation is a feature of disease progression in HCC.MACC1 mRNA expression in the HCC may become an independent predictive factor for recurrence and survival in postoperative HCC patients.
