CAJ | 학술논문

目的建立ITS(包括ITSI-5.8 rRNA-ITS2)测序鉴定真菌性鼻窦炎病原的方法.方法收集北京同仁医院2006-2008年,经临床与CT诊断为真菌性鼻窦炎,并行鼻内镜手术切除的组织标本270份.所有标本分别进行组织病理检查、压片直接镜检、真菌培养鉴定和核糖体RNA转录间隔区测序分析,通过方法比较,评价序列分析直接鉴定病原真菌的可行性,同时分析真菌性鼻窦炎病原学特征.结果在270份标本中,组织病理阳性率为80.0%(216/270),压片阳性率为80.0%(216/270),真菌培养阳性率为53.0%(143/270),ITS测序阳性率为63.0%(170/270).经培养得到22个种,6个属.ITS测序鉴定32个种.培养与ITS序列种水平符合率为76.1%(102/143).结论 ITS测序可成为真菌鉴定的辅助工具.
목적 건립ITS(포괄ITSI-5.8 rRNA-ITS2)측서감정진균성비두염병원적방법.방법 수집북경동인의원2006-2008년,경림상여CT진단위진균성비두염,병행비내경수술절제적조직표본270빈.소유표본분별진행조직병리검사、압편직접경검、진균배양감정화핵당체RNA전록간격구측서분석,통과방법비교,평개서렬분석직접감정병원진균적가행성,동시분석진균성비두염병원학특정.결과 재270빈표본중,조직병리양성솔위80.0%(216/270),압편양성솔위80.0%(216/270),진균배양양성솔위53.0%(143/270),ITS측서양성솔위63.0%(170/270).경배양득도22개충,6개속.ITS측서감정32개충.배양여ITS서렬충수평부합솔위76.1%(102/143).결론 ITS측서가성위진균감정적보조공구.
Objective To establish a molecular technique of internal transcribed spacer (ITS) sequencing to identify pathogenic fungi species from the fungal sinusitis tissues. Methods Total 270 sinusitis tissues samples were collected by endoscopic surgery from 2006 to 2008. The histopathology, organize spring clip culturation and ITS region (ITS region region of fungal rRNA, including ITS1-5. 8S rRNA-ITS2) sequencing were employed simultaneously. And then to evaluate the ITS sequencing as the tool for identification of pathogenic fungi directly from clinical samples. Results Of the 270 samples, histopathology positive rate was 80.0% (216/270) , organize spring clip positive rate was 80.0% (216/ 270), fungal culturation positive rate was 53.0% (143/270) , ITS region sequencing positive rate was 63. 0% [ (134 +28 +8)/270], There were 22 species and 6 genera identified by fungal culturation, and 32 species identified by ITS region sequencing. Conclusion ITS region sequencing will become a applicable tool in clinical laboratory in future.