CAJ | 학술논문

用荧光分光光度法研究了盐酸洛美沙星在血清中的荧光光谱特性，并对其在血清样品中的含量进行了测定．用乙腈沉淀蛋白对血清进行预处理，在硼酸盐缓冲溶液中，用硫酸调节酸度，并使其pH值为3．0，以β环糊精为荧光增敏剂．通过扫描其激发光谱和发射光谱，可知盐酸洛美沙星在血清中有以下荧光特性：盐酸洛美沙星血清提取液和盐酸洛美沙星的标准溶液具有相同特征的激发光谱和发射光谱，其激发波长为288nm，发射波长为450nm，也即药物光谱不受血清成分干扰，但血清样品对盐酸洛美沙星的荧光强度造成强烈的荧光猝灭．测定血清中盐酸洛美沙星的线性范围为：0．v5～5．0μg·ml，其回归方程为：y＝-0．237 0+4．809X(R＝0．996 8)，血清中盐酸洛美沙星的平均回收率为：99．5％，方法的相对标准偏差为：0．82％(n＝5)．
용형광분광광도법연구료염산락미사성재혈청중적형광광보특성，병대기재혈청양품중적함량진행료측정．용을정침정단백대혈청진행예처리，재붕산염완충용액중，용류산조절산도，병사기pH치위3．0，이β배호정위형광증민제．통과소묘기격발광보화발사광보，가지염산락미사성재혈청중유이하형광특성：염산락미사성혈청제취액화염산락미사성적표준용액구유상동특정적격발광보화발사광보，기격발파장위288nm，발사파장위450nm，야즉약물광보불수혈청성분간우，단혈청양품대염산락미사성적형광강도조성강렬적형광졸멸．측정혈청중염산락미사성적선성범위위：0．v5～5．0μg·ml，기회귀방정위：y＝-0．237 0+4．809X(R＝0．996 8)，혈청중염산락미사성적평균회수솔위：99．5％，방법적상대표준편차위：0．82％(n＝5)．
A method of florescence spectrophotometry for study of fluorescence spectrum characteristic and determination of lomefloxacin hydrochloride is developed in serum by florescence spectrophotometry. Acetonitrile is used to remove protein in the serum. The pH of the system is controlled at 3.0 in borate and vitriol buffer and the β-CD is added to boost up fluorescence. Through scanning excitation and emission spectrum, the fluorescence spectrophotometry of lomefloxacin hydrochloride in serum is known as follow: extracting solution of lomefloxacin hydrochloride in serum and standard solution of lomefloxacin hydrochloride owns the same character of excitation and emission spectrum, λex,m＝288nm, λem,m＝450nm. That is the spectrum of the drug is not distrubed by the components of serum, but the serum can quench lomefloxacin hydrochloride. The linear range is 0. 005～5.0μg/ml and regression equation is Y=-0. 237 0+4. 809X(R=0. 996 8). The average recovery of lomefloxacin hydrochloride is 99. 5% in serum. The relative standard deviation of the method is 0.82%(n=5).