CAJ | 학술논문

[目的]为了给牛体细胞克隆和转基因克隆工作提供基础性材料.[方法]在显微镜下,从卵液中捡出卵丘-卵母细胞复合体,置入成熟培养液中进行成熟培养,处理培养不同时期的卵母细胞,所得的去掉卵丘的卵母细胞固定在载玻片上,染色、冲洗、晾干,在显微镜下观察.[结果]表明:成熟培养0 h到4 h大多数卵母细胞处于GV期(97.5%～87.8%),培养6 h以后GVBD发生了卵母细胞数量明显增多(51.6%),成熟培养8 h～12 h处于Pre-MI的卵母细胞逐渐减少(76.7%～43.2%),MI期卵母细胞逐渐增多(13.3%～50.0%).成熟培养16 h有17.8%的卵母细胞处于MII期,大部分细胞仍处于MI期(40.0%)和AI/TI期(42.2%).从16 h～24 h,MII期卵逐渐增多,培养24 h后大多数卵母细胞排出第一极体到达MII期(86.5%).[结论]在减数分裂过程中,染色体也发生了显著的形态变化.第一次减数分裂中期时的染色体清晰可数,进入后期时,分开的两团染色体各自凝集成染色质团,并且一直持续到末期,到达第二次减数分裂中期时又成为清晰可数的染色体状态.
[목적]위료급우체세포극륭화전기인극륭공작제공기출성재료.[방법]재현미경하,종란액중검출란구-란모세포복합체,치입성숙배양액중진행성숙배양,처리배양불동시기적란모세포,소득적거도란구적란모세포고정재재파편상,염색、충세、량간,재현미경하관찰.[결과]표명:성숙배양0 h도4 h대다수란모세포처우GV기(97.5%～87.8%),배양6 h이후GVBD발생료란모세포수량명현증다(51.6%),성숙배양8 h～12 h처우Pre-MI적란모세포축점감소(76.7%～43.2%),MI기란모세포축점증다(13.3%～50.0%).성숙배양16 h유17.8%적란모세포처우MII기,대부분세포잉처우MI기(40.0%)화AI/TI기(42.2%).종16 h～24 h,MII기란축점증다,배양24 h후대다수란모세포배출제일겁체도체MII기(86.5%).[결론]재감수분렬과정중,염색체야발생료현저적형태변화.제일차감수분렬중기시적염색체청석가수,진입후기시,분개적량단염색체각자응집성염색질단,병차일직지속도말기,도체제이차감수분렬중기시우성위청석가수적염색체상태.
To investigate the meiotic progression and the chromosomal morphological changes of the bovine oocytes matured in vitro, oocytes were collected form from ooctye liquid under microscope. The majority of the oocytes maintained at GV stage (97.5%～87.8%) during the first 4 h after culture. Six hours later, around 51.6% of the oocytes occurred GVBD. When the oocytes were matured for 8h to 12h, the number of the oocytes in pre-metaphase stage gradually decreased (from 76.7% to 43.2%) and the oocytes in metaphase I (MI) rapidly increased (from 13.3% to 50.0%). Approximately 17.8% of the oocytes reached metaphase II (MII) at 16 h, and reached the highest (86.5%) after the oocytes matured for 24 h. Simultaneously, the morphology and configuration of the chromosomes occurred obviously changed during in vitro maturation. The chromosomes were accountable at MI, and gradually became condensed into mass of chromatin when the oocytes developed to anaphase and telophase. The chromosomes then became countable when the oocytes developed to next metaphase stage.