CAJ | 학술논문

利用L_9(3~4)正交试验设计表,采用绿原酸、咖啡酸、异槲皮苷三种单体为含量测定指标,同时考虑总固体物得率,探讨了返魂草提取工艺中的几个影响因素,并对实验结果进行极差和方差分析.实验结果表明,提取次数为3次、90℃条件下、乙醇质量分数为60%、每次提取6 h为最佳提取工艺.该工艺操作简便、稳定可靠,为返魂草新制剂的研究提供了参考.
이용L_9(3~4)정교시험설계표,채용록원산、가배산、이곡피감삼충단체위함량측정지표,동시고필총고체물득솔,탐토료반혼초제취공예중적궤개영향인소,병대실험결과진행겁차화방차분석.실험결과표명,제취차수위3차、90℃조건하、을순질량분수위60%、매차제취6 h위최가제취공예.해공예조작간편、은정가고,위반혼초신제제적연구제공료삼고.
A reasonable extraction process to the chemical constituents of Senecio cannabifolius Less. was designed. Method of L_9(3~4) orthogonal design was employed with content of effective constituents chlorogenic acid, caffeic acid, isoquercitrin as assaying investigating indexes first time and regarding total solids yield simultaneously. The three compounds' content was determinated by RP-HPLC and approached several influencing factors of extracting technology of Senecio cannabifolius Less.. Test result was detected with range analysis and variance analysis .Optimum extraction process consisted of refluxing for 3 times, at 90℃, with 60% ethanol and 6 h each time. Such a convenient procedure is steady, reliable, and provides base for its new preparation.