CAJ | 학술논문

目的:建立参芪颗粒(人参、黄芪、白术、茯苓等)中人参皂苷Rb_1、Rc、Rb_2与Rd的含量测定方法.方法:采用微管液相色谱梯度洗脱法,色谱柱为Microsil C_(18)微管色谱柱(150 mm×1.0 am,3 μm),流动相:乙腈-水梯度洗脱,流速:50μL/min,检测波长为203 nm.结果:人参皂苷Rb_1在0.24～2.40μg范围内呈良好线件关系(r=0.999 9),平均回收率为98.14%,RSD=0.67%(n=6);人参皂苷Rc在0.20～2.013μg范围内旱良好线性关系(r=0.999 9),平均回收率为98.04%,RSD=0.99%(n=6);人参皂苷Rb_2在0.11～1.10 μg范围内呈良好线性关系(r=0.999 4),平均同收率为98.79%,RSD=0.75%(n=6);人参皂苷Rd在0.025～0.50 μg范围内呈良好线性关系(r=0.999 1),平均回收率为97.97%,RSD=1.46%(n=6).结论:本法简便、准确,能够用于该制剂的质量控制.
목적:건립삼기과립(인삼、황기、백술、복령등)중인삼조감Rb_1、Rc、Rb_2여Rd적함량측정방법.방법:채용미관액상색보제도세탈법,색보주위Microsil C_(18)미관색보주(150 mm×1.0 am,3 μm),류동상:을정-수제도세탈,류속:50μL/min,검측파장위203 nm.결과:인삼조감Rb_1재0.24～2.40μg범위내정량호선건관계(r=0.999 9),평균회수솔위98.14%,RSD=0.67%(n=6);인삼조감Rc재0.20～2.013μg범위내한량호선성관계(r=0.999 9),평균회수솔위98.04%,RSD=0.99%(n=6);인삼조감Rb_2재0.11～1.10 μg범위내정량호선성관계(r=0.999 4),평균동수솔위98.79%,RSD=0.75%(n=6);인삼조감Rd재0.025～0.50 μg범위내정량호선성관계(r=0.999 1),평균회수솔위97.97%,RSD=1.46%(n=6).결론:본법간편、준학,능구용우해제제적질량공제.
AIM: To develop a method for determining ginsenoside Rb_1,Rc,Rb_2 and Rd in Shenqi Granules (Radix Rhizoma Ginseng,Radix Astragali,Rhizoma Atractylodis macrocephalae,Poria,etc.) by microbore LC.METHODS: Microbore liquid chromatography was applied.Microsil C_(18) column( 150 mm×1.0 mm) was used with the mobile phase containing acetonitrile-water for gradient elution.The flow rate was 50 μL/min,and the detection wavelength was set at 220 nm.RESULTS : The relationships between the concentrations and the peak areas of these for components were all linear.The recoveries were 98.14% for ginsenoside Rb_1,98.04% for ginsenoside Rc,98.79% for ginsenoside Rb_2,and 97.97% for ginsenoside Rd respectively.The relative standard deviations (RSD) were 0.67%,0.99%,0.75%,and 1.46%,respectively.CONCLUSION: This method is simple,convenient and can be used for quality control.