CAJ | 학술논문

目的研究肾病综合征患儿血清抗增殖蛋白(prohibitin,PHB)蛋白水平及其在肾小管间质早期损伤中的意义.方法应用Western blot对36例原发性肾病综合征患儿血清进行PHB蛋白水平检测,同期正常体检儿童30例为对照组.同时检测两组血清肌酐(Scr)、血尿素氮(BUN)、尿微量蛋白系列白蛋白/肌酐(ALBU/Cr)、N-乙酰β-D-葡萄糖苷酶/肌酐(NAGU/Cr)、免疫球蛋白G/肌酐(IgGU/Cr)、α1微球蛋白/肌酐(α1-MU/Cr)等.肾小球及肾小管间质损伤评分参照Katafuchi半定量法进行.结果正常对照组儿童血中未检测到PHB蛋白,肾病综合征患儿血PHB蛋白水平不同程度增高(0.203 ± 0.032 比 0 ± 0,P ＜ 0.05).伴增生性病变肾病综合征患儿血PHB水平明显高于非增生性患儿.血PHB水平与肾小管间质损伤程度以及肾小球损伤程度均呈明显正相关(r ＝ 0.868、0.753,P均＜ 0.001);患儿血PHB水平与尿微量蛋白NAG、IgG呈正相关(r ＝ 0.586、0.341,P均＜ 0.001).结论肾脏疾病患儿血PHB表达明显增高,而且血PHB水平与肾小球及肾小管间质损伤程度明显相关.血PHB水平测定可作为肾脏损伤的早期临床指标.
목적 연구신병종합정환인혈청항증식단백(prohibitin,PHB)단백수평급기재신소관간질조기손상중적의의.방법 응용Western blot대36례원발성신병종합정환인혈청진행PHB단백수평검측,동기정상체검인동30례위대조조.동시검측량조혈청기항(Scr)、혈뇨소담(BUN)、뇨미량단백계렬백단백/기항(ALBU/Cr)、N-을선β-D-포도당감매/기항(NAGU/Cr)、면역구단백G/기항(IgGU/Cr)、α1미구단백/기항(α1-MU/Cr)등.신소구급신소관간질손상평분삼조Katafuchi반정량법진행.결과 정상대조조인동혈중미검측도PHB단백,신병종합정환인혈PHB단백수평불동정도증고(0.203 ± 0.032 비 0 ± 0,P ＜ 0.05).반증생성병변신병종합정환인혈PHB수평명현고우비증생성환인.혈PHB수평여신소관간질손상정도이급신소구손상정도균정명현정상관(r ＝ 0.868、0.753,P균＜ 0.001);환인혈PHB수평여뇨미량단백NAG、IgG정정상관(r ＝ 0.586、0.341,P균＜ 0.001).결론 신장질병환인혈PHB표체명현증고,이차혈PHB수평여신소구급신소관간질손상정도명현상관.혈PHB수평측정가작위신장손상적조기림상지표.
Objective To detect the serum prohibitin protein(PHB)level in children with renal interstitial damage and analyze the correlation between PHB and renal interstitial fibrosis(RIF). Methods Serum PHB protein levels were determined by Western blot analysis in 36 children with kidney diseases,and 30 healthy children were studied as control. Levels of BUN,Scr,and urinary microprotein series(including ALBU/Cr,NAGU/Cr,IgG U/Cr,α1-MU/Cr)were studied by automatic biochemical analyzer. Renal interstitial damage was semiquantitatively graded according to Katafuchi's method. The correlation between serum levels of serum PHB protein and those of BUN,Scr as well as urine microprotein were analyzed. Results Serum PHB protein was positive in children with diverse kidney diseases however it was negative in the normal controls(P ＜ 0.05). Serum PHB levels were significantly higher in children with proliferative glomerulonephritis than those with non-proliferative glomerulonephritis(P ＜ 0.05). Statistical analysis indicated that serum PHB levels positively correlated with the degree of tubulointerstitial lesions(r ＝ 0.868,P ＜ 0.001)as well as the glomerular injuries(r ＝ 0.753,P ＜ 0.001). And,serum PHB levels were also positively correlated with urinary microprotein including NAG(r ＝ 0.586,P ＜ 0.001)and IgG(r ＝ 0.341,P ＜ 0.001). Conclusions Serum PHB levels were significantly increased in children with kidney diseases and were positively correlated with the degrees of renal interstitial damage,suggesting that PHB might be a potential clinical marker for detecting tubulointerstitial lesions.