CAJ | 학술논문

为分析玉米着丝粒卫星DNA(CentC)和着丝粒反转录转座子(CRM)在玉米种的亚种及近缘种中的保守性.采用双色荧光原位杂交检测了这两种重复序列在墨西哥玉米、二倍体多年生类玉米、多年生类玉米、摩擦禾、薏苡、高梁中的存在和分布.CentC、CRM探针在墨西哥玉米、二倍体多年生类玉米和多年生类玉米的所有染色体的着丝粒区产生了强或较强的杂交信号,而且不同染色体的杂交信号的强度存在差异,表明两种玉米着丝粒重复序列在不同着丝粒中的数量不同;此外,部分着丝粒中的CentC和CRM信号的强度存在差异.不完全重叠.CentC、CRM探针仅在摩擦禾的多数染色体的着丝粒区产生了弱的杂交信号.在薏苡和高梁中仅测检到主要分布在着丝粒区的较强或强的CRM信号.这些结果表明,CentC在玉米种的亚种间及玉蜀黍属的物种间高度保守,在与玉蜀黍属亲缘关系最近的摩擦禾属物种中也具有较高的保守性;CRM在与玉蜀黍属亲缘关系较近和较远的禾本科种属中都具有保守性.
위분석옥미착사립위성DNA(CentC)화착사립반전록전좌자(CRM)재옥미충적아충급근연충중적보수성.채용쌍색형광원위잡교검측료저량충중복서렬재묵서가옥미、이배체다년생류옥미、다년생류옥미、마찰화、의이、고량중적존재화분포.CentC、CRM탐침재묵서가옥미、이배체다년생류옥미화다년생류옥미적소유염색체적착사립구산생료강혹교강적잡교신호,이차불동염색체적잡교신호적강도존재차이,표명량충옥미착사립중복서렬재불동착사립중적수량불동;차외,부분착사립중적CentC화CRM신호적강도존재차이.불완전중첩.CentC、CRM탐침부재마찰화적다수염색체적착사립구산생료약적잡교신호.재의이화고량중부측검도주요분포재착사립구적교강혹강적CRM신호.저사결과표명,CentC재옥미충적아충간급옥촉서속적물충간고도보수,재여옥촉서속친연관계최근적마찰화속물충중야구유교고적보수성;CRM재여옥촉서속친연관계교근화교원적화본과충속중도구유보수성.
In order to analyze the conservation of maize centromeric satellite DNA (CentC) and centromeric retrotransposon (CRM) in the subspecies and relatives of Zea mays,dual fluorescence in situ hybridization (FISH) was used to detect the existence and distribution of the above two repetitive sequences in Zea mays ssp.mexicana,Z.diploperennis,Z.perennis,Tripsacum dactyloides,Coix lacryma-jobi,and Sorghum bicolor.In Z.mays ssp.mexicana,Z.diploperennis,and Z.perennis,both CentC and CRM probes produced strong or relatively strong signals in the centromeric regions of all chromosomes.There was an obvious variation in the intensity of hybridization signals on different chromosomes,indicating that different centromeres have different amounts of CentC and CRM sequences.In some centromeres,the intensity of CentC signals differed from that of CRM signals and was free from overlapping.In T.dactyloides,only weak CentC and CRM signals were detected in the centromeric regions of most chromosomes,while in C lacryma-jobi and S.bicolor only relatively strong or strong CRM signals primarily located in the centromeric regions were detected.This result indicates that CentC is highly conserved among the subspecies of Z.mays and the species of Zea,and has high conservation in Tripsacum,a genus that is most closely related to Zea,and CRM is conserved among the species of grass family either closely or distantly related to Zea.