CAJ | 학술논문

本研究旨在阐明猬迭宫绦虫湖南分离株线粒体细胞色素c氧化酶第I亚基(cox1)基因部分序列(pcox1)和烟酰胺腺嘌呤二核苷酸(NADH)脱氢酶亚单位1基因(nad1)部分序列(pnad1)的遗传变异情况,并用pcox1和pnad1序列重构猬迭宫绦虫与其它绦虫的种群遗传关系.利用聚合酶链反应(PCR)扩增猬迭宫绦虫的pcox1和pnad1,应用ClustalX 1.81程序对序列进行比对,再用Phylip3.67程序MP法和Mage4.0程序NJ法绘制种系发育树,并用Puzzle5.2程序构建最大似然树,同时利用DNAStar5.0中的Megalign程序进行同源性分析.结果显示所获得各样品株的pcox1和pnad1序列长度一致,分别为396和566 bp,湖南分离株与已知猬迭宫绦虫位于同一分枝.由于猬迭宫绦虫pcox1和pnad1序列种内相对保守,种间差异较大,故均可作为种间遗传变异研究的标记,从而为猬迭官绦虫的种群体遗传学研究和其相关疾病的诊断奠定基础.
본연구지재천명위질궁조충호남분리주선립체세포색소c양화매제I아기(cox1)기인부분서렬(pcox1)화연선알선표령이핵감산(NADH)탈경매아단위1기인(nad1)부분서렬(pnad1)적유전변이정황,병용pcox1화pnad1서렬중구위질궁조충여기타조충적충군유전관계.이용취합매련반응(PCR)확증위질궁조충적pcox1화pnad1,응용ClustalX 1.81정서대서렬진행비대,재용Phylip3.67정서MP법화Mage4.0정서NJ법회제충계발육수,병용Puzzle5.2정서구건최대사연수,동시이용DNAStar5.0중적Megalign정서진행동원성분석.결과현시소획득각양품주적pcox1화pnad1서렬장도일치,분별위396화566 bp,호남분리주여이지위질궁조충위우동일분지.유우위질궁조충pcox1화pnad1서렬충내상대보수,충간차이교대,고균가작위충간유전변이연구적표기,종이위위질관조충적충군체유전학연구화기상관질병적진단전정기출.
The objectives of the present study were to examine sequence variation in the mitochondrial cytochrome coxidase subunit 1 (coxl) gene and NADH dehydrogenase subunit l(nad1) gene among Spirometra erinaceieuropaei isolates from Hunan Province,and to reconstruct their phylogenetic relationship using coxl and nadl sequences.The partial coxl (pcox1) and nadl (pnadl) was amplified from each S.erinaceieuropaei sample,and pcox1 and pnad1 sequences were aligned using the ClustalX 1.81.MP and NJ trees of pcoxl and pnad1 were constructed using the software Phylip 3.67 version 4.0 and Mage version 4.0,and ML tree was also constructed using Puzzle version 5.2.Sequence homology analysis was performed using the Megalign program of the software DNAStar version 5.0.The results showed that the lengths of pcox1 and pnad1 sequences were 396 and 566 bp,respectively.The constructed phylogenetic tree revealed that the Hunan isolates and the S.erinaceieuropaei available in GenBank were clustered in the same clade.There is no significant variation in pcoxl sequences within S.erinaceieuropaei,while inter-species difference is obvious.It is concluded that pcoxl and pnad1 sequences can be used as genetic marker for population genetic studies of cestodes.The results of the present study provided foundation for further studies of population genetics of S. erinaceieuropaei,and for diagnosis of the resultant disease.