CAJ | 학술논문

采用水蒸气蒸馏法提取侧柏叶精油,通过GC和GC-MS分析精油的组成.从侧柏叶精油中鉴定出29种成分,主要为单萜和倍半萜,含量较高的化合物有:8-丙氧基-柏木烷(15.41％)、松油烯-4-醇(12.98％)、α-蒎烯(9.85％)、桧烯(9.92％)和3-蒈烯(6.77％).侧柏叶精油具有广谱的抗细菌活性,对供试细菌的最低杀菌浓度(MBC)在0.1 mg/mL和1.0 mg/mL之间,最低抑菌浓度(MIC)在0.063 mg/mL和0.90 mg/mL之间,半抑制浓度(IC50)在0.044 mg/mL和0.763 mg/mL之间.侧柏叶精油能有效抑制菲啰嗪与Fe2+的螯合(IC50值为0.409 mg/mL)和β-胡萝卜素-亚油酸的氧化(IC50值为0.526mg/mL).
채용수증기증류법제취측백협정유,통과GC화GC-MS분석정유적조성.종측백협정유중감정출29충성분,주요위단첩화배반첩,함량교고적화합물유:8-병양기-백목완(15.41％)、송유희-4-순(12.98％)、α-파희(9.85％)、회희(9.92％)화3-개희(6.77％).측백협정유구유엄보적항세균활성,대공시세균적최저살균농도(MBC)재0.1 mg/mL화1.0 mg/mL지간,최저억균농도(MIC)재0.063 mg/mL화0.90 mg/mL지간,반억제농도(IC50)재0.044 mg/mL화0.763 mg/mL지간.측백협정유능유효억제비라진여Fe2+적오합(IC50치위0.409 mg/mL)화β-호라복소-아유산적양화(IC50치위0.526mg/mL).
The leaf essential oil of Platycladus orientalis obtained by hydro-distillation was investigated by GC and GCMS.8-Propoxy cedrane ( 15.41％ ),terpinen-4-ol ( 12.98％ ),α-pinene (9.85％),sabenene (9.92％),and 3-carene (6.77％) were the major compounds of the 29 identified components which accounted for 96.64％ of the total oil of P.orientalis.The oil was rich in monoterpenoids and sesquiterpenoids.It was assayed to have the broad spectral antibacterial activity with the MBC values ranged from 0.10 mg/mL to 1.0 mg/mL,the MIC values from 0.063 mg/mL to 0.90mg/mL,and the IC50 values from 0.044 mg/mL to 0.763 mg/mL.The essential oil was subjected to the screening for the antioxidant activity by ferrozine-Fe2+ complex formation and β-carotene bleaching inhibition assays.It inhibited both the formation of ferrozine-Fe2 + complex and β-carotene-linoleic acid oxidation in a dose dependent manner with the IC50 values of 0.409 mg/mL and 0.526 mg/mL,respectively.