国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2010年
6期
472-475
,共4页
马晓波%黄家禹%洪强%宋秀宇%黄朝阳%郑燕青
馬曉波%黃傢禹%洪彊%宋秀宇%黃朝暘%鄭燕青
마효파%황가우%홍강%송수우%황조양%정연청
结核病%CD4+CD25high调节性T细胞%Foxp3+
結覈病%CD4+CD25high調節性T細胞%Foxp3+
결핵병%CD4+CD25high조절성T세포%Foxp3+
Tuberculosis%CD4 + CD25high regulatory T cell%Foxp3 +
目的 分析不同结核病患者体内CD4+CD25+Foxp3+调节性T细胞(Tr)表达的变化,探讨其在结核病免疫中的作用.方法 对33例结核病患者以及同期30例健康体检者运用流式细胞术检测其外周血CD4+CD25high和CD4+CD25+Foxp3+Tr表达情况.结果 结核组CD4+CD25high和CD4+CD25+Foxp3+Tr检测结果分别为(8.84±2.55)%、(6.30±1.38)%,高于对照组(7.09±1.09)%、(5.22±0.64)%,差别有统计学意义(t=3.57,4.01,P＜0.01);痰涂阳患者CD4+CD25high和CD4+CD25+Foxp3+Tr检测结果分别为(10.52±3.27)%、(7.18±1.77)%,高于痰涂阴患者(8.21±1.94)%、(5.97±1.06)%,两组间差别均具有统计学意义(t=2.51,2.42,P＜0.05);初治患者和复治患者间CD4+CD25high和CD4+CD25+Foxp3+检测结果无统计学意义(t=0.03,0.02,P＞0.05).结论 结核病患者体内CD4+CD25high和CD4+CD25+Foxp3+Tr检测结果高于健康人群,提示患者体内存在免疫系统抑制状态.
目的 分析不同結覈病患者體內CD4+CD25+Foxp3+調節性T細胞(Tr)錶達的變化,探討其在結覈病免疫中的作用.方法 對33例結覈病患者以及同期30例健康體檢者運用流式細胞術檢測其外週血CD4+CD25high和CD4+CD25+Foxp3+Tr錶達情況.結果 結覈組CD4+CD25high和CD4+CD25+Foxp3+Tr檢測結果分彆為(8.84±2.55)%、(6.30±1.38)%,高于對照組(7.09±1.09)%、(5.22±0.64)%,差彆有統計學意義(t=3.57,4.01,P＜0.01);痰塗暘患者CD4+CD25high和CD4+CD25+Foxp3+Tr檢測結果分彆為(10.52±3.27)%、(7.18±1.77)%,高于痰塗陰患者(8.21±1.94)%、(5.97±1.06)%,兩組間差彆均具有統計學意義(t=2.51,2.42,P＜0.05);初治患者和複治患者間CD4+CD25high和CD4+CD25+Foxp3+檢測結果無統計學意義(t=0.03,0.02,P＞0.05).結論 結覈病患者體內CD4+CD25high和CD4+CD25+Foxp3+Tr檢測結果高于健康人群,提示患者體內存在免疫繫統抑製狀態.
목적 분석불동결핵병환자체내CD4+CD25+Foxp3+조절성T세포(Tr)표체적변화,탐토기재결핵병면역중적작용.방법 대33례결핵병환자이급동기30례건강체검자운용류식세포술검측기외주혈CD4+CD25high화CD4+CD25+Foxp3+Tr표체정황.결과 결핵조CD4+CD25high화CD4+CD25+Foxp3+Tr검측결과분별위(8.84±2.55)%、(6.30±1.38)%,고우대조조(7.09±1.09)%、(5.22±0.64)%,차별유통계학의의(t=3.57,4.01,P＜0.01);담도양환자CD4+CD25high화CD4+CD25+Foxp3+Tr검측결과분별위(10.52±3.27)%、(7.18±1.77)%,고우담도음환자(8.21±1.94)%、(5.97±1.06)%,량조간차별균구유통계학의의(t=2.51,2.42,P＜0.05);초치환자화복치환자간CD4+CD25high화CD4+CD25+Foxp3+검측결과무통계학의의(t=0.03,0.02,P＞0.05).결론 결핵병환자체내CD4+CD25high화CD4+CD25+Foxp3+Tr검측결과고우건강인군,제시환자체내존재면역계통억제상태.
Objective To investigate the expression of CD4 + CD25 + Foxp3 + regulatory T cell in patients with tuberculosis, and to discover its role in the immune response to mycobacterium tuberculosis. Methods Thirty-three patients with tuberculosis and 30 healthy controls were selected who were consulted in our hospital. Patients were classified by their chemotherapy and smear sputum and CD4 + CD25 + Foxp3 + regulatory T cell were detected by flow cytometry. Results Expression of CD4 + CD25high and CD4+ CD25 + Foxp3 + regulatory T cell in experimental group were ( 8.84 ± 2.55 ) % and (6.30 ± 1.38 ) % respectively, which were significantly higher than they were in control group (t = 3.57,4. 01, P ＜ 0. 01 ). The expression of CD4 + CD25high and CD4+ CD25 + Foxp3 + regulatory T cell in patients with positive smear sputum were also significant higher than that in patients with negative smear sputum ( t = 2. 51,2. 42,P ＜ 0.05). No significance was founded between the first-visit group and revisit group ( t = 0. 03, 0. 02, P ＞ 0.05 ). Conclusions CD4 + CD25high and CD4 + CD25 + Foxp3 + regulatory T cell in patients with tuberculosis were higher than healthy control, which may result in immune suppression.
