CAJ | 학술논문

目的建立HPLC测定胚宝胶囊质量的方法.方法采用HPLC测定经用2,4.二硝基氟苯衍生化后的盐酸赖氨酸的含量,色谱柱:agilentZorbax SB-C18柱(250mm×4.6mm,5μam);流动相:乙腈-0.04mol/L磷酸二氢钾溶液(40:60);流速:1.0ml/min;柱温:30℃;检测波长:360 nm.结果盐酸赖氨酸浓度在0.39～19.3μg/ml范围内有良好的线性关系(γ=0.9999)平均加样回收率为100.2%.结论本方法操作简便、结果准确、重现性好,可作为该产品的质量控制方法.
목적 건립HPLC측정배보효낭질량적방법.방법 채용HPLC측정경용2,4.이초기불분연생화후적염산뢰안산적함량,색보주:agilentZorbax SB-C18주(250mm×4.6mm,5μam);류동상:을정-0.04mol/L린산이경갑용액(40:60);류속:1.0ml/min;주온:30℃;검측파장:360 nm.결과 염산뢰안산농도재0.39～19.3μg/ml범위내유량호적선성관계(γ=0.9999)평균가양회수솔위100.2%.결론 본방법조작간편、결과준학、중현성호,가작위해산품적질량공제방법.
Objective To establish a HPLC method for quality control of Pei-bao capsule. Methods The content of lysine derivatized from 2,4-dinitoflruorobenzene was determined by HPLC, with chromatographic column being the agilent Zorbax SB-C18 column (250 mm×4.6 mm, 5 μm) , mobile phase being acetonirile-0.04mol/L, potassium dihydrogen phosphate (40 : 60), the flow rate being 1.0 ml/min, the column temperature being 30 ℃, and wavelength being 360 nm. Results It showed good linear coorelation when the concentration of Lysine hydrochloride being within 0.39～ 19.3μg/ml (γ =0.9999) , and the average sample recovery rate was 100.2%. Conclusion This method is simple, accurate, stable and reliable. It can be used for quality control of Pei-bao capsule.