中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2009年
2期
79-82
,共4页
樊剑锋%朱晓敏%于忠勤%徐建国%宣晓琪
樊劍鋒%硃曉敏%于忠勤%徐建國%宣曉琪
번검봉%주효민%우충근%서건국%선효기
淋巴细胞亚群%肠套叠%流式细胞术
淋巴細胞亞群%腸套疊%流式細胞術
림파세포아군%장투첩%류식세포술
Lymphocyte subsets%Intussusception%Flow cytometry
目的 探索肠套叠患儿细胞免疫状况的变化.方法 采用流式细胞仪检测肠套叠患儿急性期及对照组外周血T辅助淋巴细胞Th1和Th2的百分率、CD3、CIN+、CD8+、NK细胞、B细胞百分率及C1N/CD8比值.对10例肠套叠恢复期患儿复查淋巴细胞亚群.结果 肠套叠患儿急性期外周血Th1细胞百分率(15.98±9.83)%明显低于对照组(20.83±6.79)%(P<0.05);肠套叠组Th2细胞百分率(5.48±1.86)%较对照组(4.31±2.02)%明显增高(P<0.05).Th1/Th2比值,肠套叠组为3.51±2.49,对照组为5.89±3.14,二组比较有非常显著性差异(P<0.01).急性期NK细胞百分率明显降低(P<0.05),但肠套叠组B细胞、CD3、CIN+、CD8+及CD4/CD8与对照组比较均无统计学差异(P>0.05).10例肠套叠缓解期患儿淋巴细胞亚群结果表明,NK细胞百分率显著升高(P<0.05),其他各项指标在急性期与缓解期无明显差异.结论 肠套叠患儿最重要的免疫异常是Th1/Th2细胞比例和功能失衡,主要表现为Th2细胞应答优势存在,Th1/Th2显著降低.肠套叠急性期肠道抗感染免疫力下降.
目的 探索腸套疊患兒細胞免疫狀況的變化.方法 採用流式細胞儀檢測腸套疊患兒急性期及對照組外週血T輔助淋巴細胞Th1和Th2的百分率、CD3、CIN+、CD8+、NK細胞、B細胞百分率及C1N/CD8比值.對10例腸套疊恢複期患兒複查淋巴細胞亞群.結果 腸套疊患兒急性期外週血Th1細胞百分率(15.98±9.83)%明顯低于對照組(20.83±6.79)%(P<0.05);腸套疊組Th2細胞百分率(5.48±1.86)%較對照組(4.31±2.02)%明顯增高(P<0.05).Th1/Th2比值,腸套疊組為3.51±2.49,對照組為5.89±3.14,二組比較有非常顯著性差異(P<0.01).急性期NK細胞百分率明顯降低(P<0.05),但腸套疊組B細胞、CD3、CIN+、CD8+及CD4/CD8與對照組比較均無統計學差異(P>0.05).10例腸套疊緩解期患兒淋巴細胞亞群結果錶明,NK細胞百分率顯著升高(P<0.05),其他各項指標在急性期與緩解期無明顯差異.結論 腸套疊患兒最重要的免疫異常是Th1/Th2細胞比例和功能失衡,主要錶現為Th2細胞應答優勢存在,Th1/Th2顯著降低.腸套疊急性期腸道抗感染免疫力下降.
목적 탐색장투첩환인세포면역상황적변화.방법 채용류식세포의검측장투첩환인급성기급대조조외주혈T보조림파세포Th1화Th2적백분솔、CD3、CIN+、CD8+、NK세포、B세포백분솔급C1N/CD8비치.대10례장투첩회복기환인복사림파세포아군.결과 장투첩환인급성기외주혈Th1세포백분솔(15.98±9.83)%명현저우대조조(20.83±6.79)%(P<0.05);장투첩조Th2세포백분솔(5.48±1.86)%교대조조(4.31±2.02)%명현증고(P<0.05).Th1/Th2비치,장투첩조위3.51±2.49,대조조위5.89±3.14,이조비교유비상현저성차이(P<0.01).급성기NK세포백분솔명현강저(P<0.05),단장투첩조B세포、CD3、CIN+、CD8+급CD4/CD8여대조조비교균무통계학차이(P>0.05).10례장투첩완해기환인림파세포아군결과표명,NK세포백분솔현저승고(P<0.05),기타각항지표재급성기여완해기무명현차이.결론 장투첩환인최중요적면역이상시Th1/Th2세포비례화공능실형,주요표현위Th2세포응답우세존재,Th1/Th2현저강저.장투첩급성기장도항감염면역력하강.
Objective To investigate the alteration of cellular immunity in infants with intussusception.Methods The expression of T-assistant lymphocyte subsets in peripheral blood in infants with intussusception were investigated and compared with that in normal infants by flow cytometer,including the percentages of Thi,Th2,CD4+ cells,NK ceils,B cells,CD3,CD8+cells,and the ratio of CD4/CD8.Results The percentage of Thl(15.98±9.83)%in patients with intussusception was significantly lower than that in normalinfants(20.83±6.79)%(P<0.05).The percentage of Th2(5.48±1.86)%in patients with intussusception was significantly higher than that of normalinfants(4.31±2.02)%(P<0.05).The ratio of Th1/Th2 in patients with intussusception was 3.51±2.49,but in the control group,the ratio was 5.89±3.14,there was significant difference between two groups(P<0.01).The percentage of NK cells in patients with intussusception was significantly lower than that in control group(P<0.01).There was no difference between infants in two groups in the percentage of CD3,CD4+,CD8+cells and B cells,nor in the ratio of CD4/CD8(P>0.05).The T-lymphocyte subsets of 10 infants with intussusceDtion in the stage of remission showed that the percentage of NK cells was significantly higher than that in the acute stage of intussusceDtion(P<0.05),and the others had no difference between the two stages.Conclusions The main abnormalities are the enhancement of the cellular immunity activity of Th2 and the significantly 10wer ratio of Th1/Th2 in infants with intussception.Meanwhile,the function of anti-infection immunity decreases in the intestine of infants with acute intussusception.