中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2010年
8期
1048-1051
,共4页
受体,CXCR/代谢%骨肉瘤/代谢%细胞增殖%地诺前列酮/代谢
受體,CXCR/代謝%骨肉瘤/代謝%細胞增殖%地諾前列酮/代謝
수체,CXCR/대사%골육류/대사%세포증식%지낙전렬동/대사
Receptors,CXCR/ME%Osteosarcoma/ME%Cell proliferation%Dinoprostone/ME
目的 研究CXCR7在骨肉瘤组织和骨肉瘤周围正常组织的表达差异,并构建CXCR7腺病毒表达载体,转染骨肉瘤细胞株MG63,观察CXCR7对MG63细胞增殖、炎症因子PGE2分泌的影响.方法 RT-qPCR检测CXCR7在骨肉瘤组织、细胞和骨肉瘤周围正常组织的表达差异;构建CXCR7腺病毒表达载体,感染MG63细胞后,采用RT-qPCR、MTT、ELISA分别检测其对MG63细胞CXCR7 mRNA表达、细胞增殖和PGE2分泌的影响.结果 (1)RT-qPCR结果显示,在骨肉瘤组织CXCR7 mRNA表达率93.80%和表达量(2.221±0.112)均显著高于骨肉瘤周围正常组织45.71%、(0.3448±0.098);MG63细胞低表达CXCR7 mRNA.(2)CXCR7腺病毒表达载体转染MG63细胞48、72、96 h后,显著促进MG63细胞增殖(P<0.05)和PGE2分泌(P<0.05).结论 骨肉瘤组织CX-CR7mRNA和蛋白表达显著高于骨肉瘤周围正常组织;CXCR7表达增高可以促进骨肉瘤细胞增值,促进炎症因子PGE2分泌,对骨肉瘤的进展将产生不利的影响.
目的 研究CXCR7在骨肉瘤組織和骨肉瘤週圍正常組織的錶達差異,併構建CXCR7腺病毒錶達載體,轉染骨肉瘤細胞株MG63,觀察CXCR7對MG63細胞增殖、炎癥因子PGE2分泌的影響.方法 RT-qPCR檢測CXCR7在骨肉瘤組織、細胞和骨肉瘤週圍正常組織的錶達差異;構建CXCR7腺病毒錶達載體,感染MG63細胞後,採用RT-qPCR、MTT、ELISA分彆檢測其對MG63細胞CXCR7 mRNA錶達、細胞增殖和PGE2分泌的影響.結果 (1)RT-qPCR結果顯示,在骨肉瘤組織CXCR7 mRNA錶達率93.80%和錶達量(2.221±0.112)均顯著高于骨肉瘤週圍正常組織45.71%、(0.3448±0.098);MG63細胞低錶達CXCR7 mRNA.(2)CXCR7腺病毒錶達載體轉染MG63細胞48、72、96 h後,顯著促進MG63細胞增殖(P<0.05)和PGE2分泌(P<0.05).結論 骨肉瘤組織CX-CR7mRNA和蛋白錶達顯著高于骨肉瘤週圍正常組織;CXCR7錶達增高可以促進骨肉瘤細胞增值,促進炎癥因子PGE2分泌,對骨肉瘤的進展將產生不利的影響.
목적 연구CXCR7재골육류조직화골육류주위정상조직적표체차이,병구건CXCR7선병독표체재체,전염골육류세포주MG63,관찰CXCR7대MG63세포증식、염증인자PGE2분비적영향.방법 RT-qPCR검측CXCR7재골육류조직、세포화골육류주위정상조직적표체차이;구건CXCR7선병독표체재체,감염MG63세포후,채용RT-qPCR、MTT、ELISA분별검측기대MG63세포CXCR7 mRNA표체、세포증식화PGE2분비적영향.결과 (1)RT-qPCR결과현시,재골육류조직CXCR7 mRNA표체솔93.80%화표체량(2.221±0.112)균현저고우골육류주위정상조직45.71%、(0.3448±0.098);MG63세포저표체CXCR7 mRNA.(2)CXCR7선병독표체재체전염MG63세포48、72、96 h후,현저촉진MG63세포증식(P<0.05)화PGE2분비(P<0.05).결론 골육류조직CX-CR7mRNA화단백표체현저고우골육류주위정상조직;CXCR7표체증고가이촉진골육류세포증치,촉진염증인자PGE2분비,대골육류적진전장산생불리적영향.
Objective To study the differences of CXCR7 expression between in osteosarcoma tissues and normal peri-osteosarcoma tissues, and observe the effect on cell proliferation and inflammatory cytokine PGE2 secretion in MG63 cells when it had been transfected with pEGFP-Adv-CXCR7 vector. Methods CXCR7 expression in osteosarcoma tissues, cells and normal peri-osteosarcoma tissues detected by RT-qPCR. The CXCR7 mRNA expression, cell proliferation and PGE2 secretion of MG63 cells after transfected with pEGFP-Adv-CXCR7 expression vector were evaluated by RT-qPCR, MTT and ELISA, respectively. Results The expression rate and quality of CXCR7 mRNA in osteosarcoma tissue were 93.80% and (2. 221 ±0. 112), which significantly higher than those of normal peri-osteosarcoma tissues 45.71% and (0. 3448 ±0.098). Low expression of CXCR7 in MG63 cells was also observed. The proliferation and PGE2 secretion of MG63 cells were significantly increased at the time point of 48, 72 and 96h after pEGFPAdv-CXCR7 expression vector transfected into MG63 cells ( P < 0. 05). Conclusion The expression of CXCR7 mRNA in osteosarcoma tissues were significantly higher than normal peri-osteosarcoma tissues. CXCR7 high expression may have a negative effect on the progress of osteosarcoma.
