CAJ | 학술논문

目的研究不同剂量X射线照射及照射后不同时间点对人肺腺癌A549细胞Pokemon基因表达的影响.方法用吸收剂量分别为2、4、6和8 Gy的X射线照射体外堵养的人肺腺癌A549细胞,2、4、8、12、24、48和72 ha,用实时定量PCR技术检测其中的Pokemon mRNA表达水平,以未照射组为对照.结果在2、4、6、8 Gy X射线照射后的早期(除2 Gy照射后的2和4 h外)Pokemon mRNA的表达降低,但在晚期(48 h以后)呈升高趋势,在大部分时间点实验组与对照组的差异有统计学意义(t=3.40～154.76,P<0.05).结论较大剂量的X射线在早期可下调A549细胞Pokemon基因mRNA的表达,诱导肿瘤细胞凋亡;但在晚期又可诱导A549细胞高表达PokemonmRNA,这可能与辐射所致A549细胞的DNA损伤修复和细胞周期调控有关.
목적 연구불동제량X사선조사급조사후불동시간점대인폐선암A549세포Pokemon기인표체적영향.방법 용흡수제량분별위2、4、6화8 Gy적X사선조사체외도양적인폐선암A549세포,2、4、8、12、24、48화72 ha,용실시정량PCR기술검측기중적Pokemon mRNA표체수평,이미조사조위대조.결과 재2、4、6、8 Gy X사선조사후적조기(제2 Gy조사후적2화4 h외)Pokemon mRNA적표체강저,단재만기(48 h이후)정승고추세,재대부분시간점실험조여대조조적차이유통계학의의(t=3.40～154.76,P<0.05).결론 교대제량적X사선재조기가하조A549세포Pokemon기인mRNA적표체,유도종류세포조망;단재만기우가유도A549세포고표체PokemonmRNA,저가능여복사소치A549세포적DNA손상수복화세포주기조공유관.
Objective To study the dose-and time-effects of X-ray irradiation on the expression of Pokemon gene in A549 cells of human lung adenocarcinoma.Methods A549 cells were cultured in vitro and exposed to X-rays with the doses of 2,4,6 and 8 Gy,respectively.Untreated A549 cells were used as control group.The relative levels of Pokemon mRNA expression in the cells were detected by using quantitative real-time PCR at 2,4,8,12,24,48 and 72 h after irradiation.Results The Pokemon mRNA expression levels decreased in the early period after irradiation(except 2 and 4 h after irradiation in 2 Gy group)and then increased in the later stage(48 h after irradiation)with significant statistical differences at the most time points in comparison with the control group(t=3.40-154.76,P<0.05).Conclusions Higher doses of X-rays may degrade the expression of Pokemon mRNA in the human A549 cells and induce apoptosis in the early period,hut also may upgrade its expression in the later period, which might be correlated with the cell cycle regulation and DNA damage repair in the A549 cells.