中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2008年
4期
269-271
,共3页
赵素兰%王荣华%李洁%邱雪莲%陈杰鹏%周玲%叶淑清%林京%李风生%赵华
趙素蘭%王榮華%李潔%邱雪蓮%陳傑鵬%週玲%葉淑清%林京%李風生%趙華
조소란%왕영화%리길%구설련%진걸붕%주령%협숙청%림경%리풍생%조화
维百氏康空气健康剂%流感病毒A型,人%流感病毒B型
維百氏康空氣健康劑%流感病毒A型,人%流感病毒B型
유백씨강공기건강제%류감병독A형,인%류감병독B형
"BenovoairTM Concentrate"%Influenza A virus,human%Influenza B virus
目的 进行维百氏康空气健康剂抗流感病毒实验研究方法将不同稀释度的样品与100 TCID50的病毒液等量混合,37℃中和30 min,取适量接种于含维持液的细胞孔内,一式3孔,设病毒对照、细胞对照和不同稀释度样品对照,置37℃ CO2培养箱,逐日观察CPE,当病毒对照组CPE出现++++时终止试验,测定血凝程度.结果样品原液的1∶60,1∶120,1∶240,1∶480可100%灭活甲型及乙型流感病毒.原液的1∶960和1∶1920可灭活25%～50%甲型及乙型流感病毒.结论为维百氏康空气健康剂产品的研发提供了抗流感病毒试验依据.
目的 進行維百氏康空氣健康劑抗流感病毒實驗研究方法將不同稀釋度的樣品與100 TCID50的病毒液等量混閤,37℃中和30 min,取適量接種于含維持液的細胞孔內,一式3孔,設病毒對照、細胞對照和不同稀釋度樣品對照,置37℃ CO2培養箱,逐日觀察CPE,噹病毒對照組CPE齣現++++時終止試驗,測定血凝程度.結果樣品原液的1∶60,1∶120,1∶240,1∶480可100%滅活甲型及乙型流感病毒.原液的1∶960和1∶1920可滅活25%～50%甲型及乙型流感病毒.結論為維百氏康空氣健康劑產品的研髮提供瞭抗流感病毒試驗依據.
목적 진행유백씨강공기건강제항류감병독실험연구방법장불동희석도적양품여100 TCID50적병독액등량혼합,37℃중화30 min,취괄량접충우함유지액적세포공내,일식3공,설병독대조、세포대조화불동희석도양품대조,치37℃ CO2배양상,축일관찰CPE,당병독대조조CPE출현++++시종지시험,측정혈응정도.결과양품원액적1∶60,1∶120,1∶240,1∶480가100%멸활갑형급을형류감병독.원액적1∶960화1∶1920가멸활25%～50%갑형급을형류감병독.결론위유백씨강공기건강제산품적연발제공료항류감병독시험의거.
Objective Anti-influenza virus activity of "BenovoairTM Concentrate". Methods The different dilution of samples were mixed with the same quantity of I00 TCID50 virus at 37℃ for 30 minutes. Add suitable quantity mixture in wells containing cells. Every 3 welLs were the same mode. Viruses control, cells control and samples control of different dilution were performed and set in the CO2 incubator at 37℃. CPE was observed every day. When CPE appears in viruses control as " ++++ ", stopped testing and performed the hemagglutination 25%-50% anti-influenza A and anti-influensa B activities. "BenovomrTM Concentrate" with dilution of 1:960 and 1:1920 have 25%-50% anti-influenza A and anti-influensa B activities.Conclusion The test was the proof of anti-influenza virus activities which provided for the development of "BenovoairTM Concentrate".
