CAJ | 학술논문

许旺细胞源神经营养因子对大鼠肌卫星细胞体外活性的影响
허왕세포원신경영양인자대대서기위성세포체외활성적영향
Effect of Schwann cell-derived neurotrophic factor on the biological characteristics of skeletal satellite cells in rats

万方数据

中华显微外科杂志中華顯微外科雜誌 중화현미외과잡지
Chinese Journal of Microsurgery
2012年 4期 299-302 ,共4页

邬江%朱敏%朱崇涛%陈斌%徐昕明%徐永清%欧阳钧%廖华%邱小忠%朱家恺%庞水发

오강%주민%주숭도%진빈%서흔명%서영청%구양균%료화%구소충%주가개%방수발

卫星细胞%许旺细胞源神经营养因子%增殖%分化衛星細胞%許旺細胞源神經營養因子%增殖%分化
위성세포%허왕세포원신경영양인자%증식%분화
Satellite cells%Schwann cell-derived neurotrophic factor%Proliferation%Differentiation

目的应用细胞体外培养研究体系,探讨许旺细胞源神经营养因子与成肌干细胞一卫星细胞的作用关系. 方法建立大鼠肌卫星细胞体外培养体系,制备含不同浓度的许旺细胞源神经营养因子培养基,动态观测在这些条件培养基作用下,肌卫星细胞的形态、MTT生长曲线和肌管形成率等指标的变化. 结果肌卫星细胞生长能力反应在细胞增殖和分化两方面,浓度为200 ng/ml SDNF可以促进卫星细胞增殖；浓度分别为50、100、200、400 ng/ml SDNF时肌管形成较为突出,相对增殖作用来说,可能促分化功能更为典型. 结论 SDNF对肌卫星细胞增殖和分化有一定的调控作用,相对而言促分化作用更加明显.推测其在延缓失神经肌萎缩方面有一定的作用.
목적 응용세포체외배양연구체계,탐토허왕세포원신경영양인자여성기간세포일위성세포적작용관계. 방법 건립대서기위성세포체외배양체계,제비함불동농도적허왕세포원신경영양인자배양기,동태관측재저사조건배양기작용하,기위성세포적형태、MTT생장곡선화기관형성솔등지표적변화. 결과 기위성세포생장능력반응재세포증식화분화량방면,농도위200 ng/ml SDNF가이촉진위성세포증식；농도분별위50、100、200、400 ng/ml SDNF시기관형성교위돌출,상대증식작용래설,가능촉분화공능경위전형. 결론 SDNF대기위성세포증식화분화유일정적조공작용,상대이언촉분화작용경가명현.추측기재연완실신경기위축방면유일정적작용.
Objective To study the effects of Schwann cell-derived neurotrophic factor(SDNF) on myoblast stem cells(called satellite cells,SCs) in vitro. Methods After setting up SCs culture system in vitro, SCs which treated with various SDNF concentrations culture medium were dynamically evaluated by cell morphology,MTT growth curve and fusion rate. Results The ability of SCs preceding their participation in muscle repair include proliferation and differentiation, 200 ng/ml SDNF stimulated cell proliferation more than the other medium,but 50 ng/ml,100 ng/ml,200 ng/ml,400 ng/ml SDNF made SCs differentiation significantly for their high myotube fusion rate. Conclusion SDNF can regulate the proliferation and differentiation of rat skeletal satellite cellsin vitro,but in differentiation significantly.SDNF might play a role in slowing down denervated muscle atrophy.