CAJ | 학술논문

目的分析华西医院地震伤员所分离的耐甲氧西林金黄色葡萄球菌(MRSA)特点,并与同期非灾区患者分离MRSA进行同源性比较.方法使用细菌基因组重复序列PCR(Rep-PCR)技术以及DiversiLab细菌同源性分析技术对地震住院伤员所分离出的5株MRSA与同期非灾区的住院患者分离的6株MRSA进行同源性分析.结果 11株MRSA的基因图谱分为5组(A-E组),地震伤员MRSA分布为A组1株、B组2株、C组1株以及E组1株,非地震伤员的MRSA分布为A组3株、B组1株、C组1株以及D组1株.结论地震伤员的MRSA与同期部分科室的非灾区患者的MRSA呈高度同源性.
목적 분석화서의원지진상원소분리적내갑양서림금황색포도구균(MRSA)특점,병여동기비재구환자분리MRSA진행동원성비교.방법 사용세균기인조중복서렬PCR(Rep-PCR)기술이급DiversiLab세균동원성분석기술대지진주원상원소분리출적5주MRSA여동기비재구적주원환자분리적6주MRSA진행동원성분석.결과 11주MRSA적기인도보분위5조(A-E조),지진상원MRSA분포위A조1주、B조2주、C조1주이급E조1주,비지진상원적MRSA분포위A조3주、B조1주、C조1주이급D조1주.결론 지진상원적MRSA여동기부분과실적비재구환자적MRSA정고도동원성.
Objective To investigate the DNA homology between the methicillin-resistant Staphylococcus aureus (MRSA) isolated from the wounds caused by earthquake and those from the patients from non-earthquake-stricken areas. Methods Five MRSA isolates were obtained from the wounds caused by earthquake of 5 in-patients admitted into the West China Hospital,and 6 MRSA isolates were obtained from the inpatient of the same hospital during the same period. DNA fingerprint alas was obtained by repetitive extragenic palindromic sequence-based PCR (Rep-PCR) and homolog analysis was conducted with the help of Rep-based DiversiLab Microbial Typing system. Results Five different patterns (A-E) were found among the 11 MRSA strains. The 5 strains isolated from the wounds caused by earthquake included subtype A (n=1),subtype B (n=2),subtype C (n=1),and subtype E (n=1). And the 6 stains isolated from the patient that come from non-earthquake-stricken areas included A (n = 3),subtype B (n =1),subtype C (n=1),and subtype D (n=1). Conclusion The MRSA stains isolated from the wounds caused by earthquake are highly homologous with those isolated from the patient from non-disastrous areas during the same period.