中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2010年
33期
2351-2353
,共3页
黄东%蒋凌英%徐维海%童晓嵋%竺海燕%李超%周枫%刘柳%林小娜%张松英
黃東%蔣凌英%徐維海%童曉嵋%竺海燕%李超%週楓%劉柳%林小娜%張鬆英
황동%장릉영%서유해%동효미%축해연%리초%주풍%류류%림소나%장송영
精子%顶体%胚胎发育%Y染色体%染色体缺失
精子%頂體%胚胎髮育%Y染色體%染色體缺失
정자%정체%배태발육%Y염색체%염색체결실
Sperm%Acrosome%Embryo development%Y chromosome%Chromosome deletion
目的 探讨圆头精子的受精能力、胚胎发育潜能及其遗传风险.方法 通过精子形态染色和透射电镜证实一例圆头精子症患者,其女方共获卵26颗,赠卵6颗,将20颗卵子(19颗为MII卵)行卵细胞质内单个圆头精子的显微注射,6颗赠卵行常规体外授精.测定该患者的染色体核型,同时针对Y染色体AZFa、AZFb、AZFc的sY84、sY86、sY127、sY134、sY254、sY255等6个序列标签位点(STS)进行Y染色体微缺失测定.结果 19颗MII卵子行圆头精子显微注射后有4颗正常受精并卵裂,其余均未受精和卵裂.6颗赠卵常规体外授精后全部正常受精并卵裂,受精率显著高于圆头精子(100%vs 21.1%,P＜0.01),卵裂率两者差异无统计学意义(100%vs 100%,P＞0.05).患者染色体核型正常(46,XY),未发现微缺失.女方行冻胚移植后妊娠,足月剖宫产一男孩,染色体核型正常(46,XY).受者行冻胚移植后妊娠,足月剖宫产一男孩,体健.结论 尽管受精率低下,卵细胞质内单精子注射(ICSI)仍不失为治疗圆头精子症患者不育的一种有效方法.
目的 探討圓頭精子的受精能力、胚胎髮育潛能及其遺傳風險.方法 通過精子形態染色和透射電鏡證實一例圓頭精子癥患者,其女方共穫卵26顆,贈卵6顆,將20顆卵子(19顆為MII卵)行卵細胞質內單箇圓頭精子的顯微註射,6顆贈卵行常規體外授精.測定該患者的染色體覈型,同時針對Y染色體AZFa、AZFb、AZFc的sY84、sY86、sY127、sY134、sY254、sY255等6箇序列標籤位點(STS)進行Y染色體微缺失測定.結果 19顆MII卵子行圓頭精子顯微註射後有4顆正常受精併卵裂,其餘均未受精和卵裂.6顆贈卵常規體外授精後全部正常受精併卵裂,受精率顯著高于圓頭精子(100%vs 21.1%,P＜0.01),卵裂率兩者差異無統計學意義(100%vs 100%,P＞0.05).患者染色體覈型正常(46,XY),未髮現微缺失.女方行凍胚移植後妊娠,足月剖宮產一男孩,染色體覈型正常(46,XY).受者行凍胚移植後妊娠,足月剖宮產一男孩,體健.結論 儘管受精率低下,卵細胞質內單精子註射(ICSI)仍不失為治療圓頭精子癥患者不育的一種有效方法.
목적 탐토원두정자적수정능력、배태발육잠능급기유전풍험.방법 통과정자형태염색화투사전경증실일례원두정자증환자,기녀방공획란26과,증란6과,장20과란자(19과위MII란)행란세포질내단개원두정자적현미주사,6과증란행상규체외수정.측정해환자적염색체핵형,동시침대Y염색체AZFa、AZFb、AZFc적sY84、sY86、sY127、sY134、sY254、sY255등6개서렬표첨위점(STS)진행Y염색체미결실측정.결과 19과MII란자행원두정자현미주사후유4과정상수정병란렬,기여균미수정화란렬.6과증란상규체외수정후전부정상수정병란렬,수정솔현저고우원두정자(100%vs 21.1%,P＜0.01),란렬솔량자차이무통계학의의(100%vs 100%,P＞0.05).환자염색체핵형정상(46,XY),미발현미결실.녀방행동배이식후임신,족월부궁산일남해,염색체핵형정상(46,XY).수자행동배이식후임신,족월부궁산일남해,체건.결론 진관수정솔저하,란세포질내단정자주사(ICSI)잉불실위치료원두정자증환자불육적일충유효방법.
Objective To explore the fertilizing ability, cleavage potential and inheritance risks of globozoospermia. Methods A globozoospermic patient was diagnosed by sperm morphological staining and transmission electron microscope. From his wife the investigators obtained 26 oocytes in which 6 oocytes were donated and the other 19 ( in 20) MII oocytes injected into 19 round-headed sperms. Six donated oocytes accepted in vitro fertilization (IVF). This patient's chromosome and microdeletion in AZFa, AZFb and AZFc in Y chromosome were checked through 6 sequence tag sites of sY84, sY86, sY127, sY134, sY254 and sY255. Results Only 4 ( in 19) were normally fertilized and cleaved. All 6 donated oocytes were normally fertilized and cleaved. The fertilizing rate was significantly higher than that of this patient( 100% vs 21.1%,P ＜0. 01 ). But the cleavage rate has no statistical difference( 100% vs 100%, P ＞0. 05). This patient had normal chromosome (46, XY) and there was no deletion in Y chromosome. His wife became pregnant after accepting two thawed embryos and then gave birth to a boy whose chromosome was normal (46, XY). The acceptor also gave birth to a healthy boy after accepting two thawed embryos. Conclusions Despite a lower fertilizing rate, intracytoplasmic sperm injection is still an effective therapy for globozoospermic infertility.
