CAJ | 학술논문

目的检测肌肉移植后肌肉萎缩盒F基因(muscle atrophy F-box,MAFbx)和肌肉环状指基因1(mLIsele ring finger 1,MuRFI)mRNA表达的变化,并探讨其与肌肉萎缩和肌肉功能的关系.方法建立大鼠股薄肌原位游离移植的模型,以自身为对照,应用real-time PCR、电生理等方法,观察MAFbx和MuRFI mRNA表达、肌肉萎缩与肌肉功能的演变,统计学分析三者之间的相关性. 结果肌肉移植术后,肌纤维横截面积、肌肉湿重维持率、肌肉最大单收缩力和肌肉强直收缩力维持率均先降低后增加,术后晚期时仍低于对照侧水平(P<0.05)6肌肉移植后MAFbx和MuRFI mRNA在术后3～4周时达到最高然后逐渐降低,最高达对照侧的7.1倍和4.1倍,术后晚期时仍持续低水平高表达,与对照侧比较差异有统计学意义(P<0.05).结论肌肉移植后MAFbx和MuRFI mRNA的持续高表达,是肌肉萎缩引起肌肉功能降低的重要联系点.二者可以作为骨骼肌萎缩早期的标记物,也可以作为药物干预的潜在靶点用于治疗肌肉萎缩.
목적 검측기육이식후기육위축합F기인(muscle atrophy F-box,MAFbx)화기육배상지기인1(mLIsele ring finger 1,MuRFI)mRNA표체적변화,병탐토기여기육위축화기육공능적관계.방법 건립대서고박기원위유리이식적모형,이자신위대조,응용real-time PCR、전생리등방법,관찰MAFbx화MuRFI mRNA표체、기육위축여기육공능적연변,통계학분석삼자지간적상관성. 결과기육이식술후,기섬유횡절면적、기육습중유지솔、기육최대단수축력화기육강직수축력유지솔균선강저후증가,술후만기시잉저우대조측수평(P<0.05)6기육이식후MAFbx화MuRFI mRNA재술후3～4주시체도최고연후축점강저,최고체대조측적7.1배화4.1배,술후만기시잉지속저수평고표체,여대조측비교차이유통계학의의(P<0.05).결론 기육이식후MAFbx화MuRFI mRNA적지속고표체,시기육위축인기기육공능강저적중요련계점.이자가이작위골격기위축조기적표기물,야가이작위약물간예적잠재파점용우치료기육위축.
Objective To study muscle atrophy F-box (MAFbx) and muscle ring finger 1 (MuRFI) mRNA expression and its relationship with muscular contraction following free muscle transfer. Methods The gracilis muscle was orthotopic transferred in adult rat to establish the animal model. The muscle at the unoperated side was used as control. The expression of MAFbx and MuRFI mRNA, the muscle contraction and muscle function were measured by real-time PCR and multiple function physiological device. The relationship among the expression of MAFbx and MuRFI mRNA, the muscle contraction and muscle function was analyzed. Results After muscle free transfer, muscle wet weight reservation, the maximum contraction and tetanus strength reduce first and increased later, but still lower than those at control side. The expression of MAFbx and MuRFI mRNA reached peak level 3 ～ 4 weeks after muscle transfer which was 7.1 and 4.1 times as that at control side. It decreased later, but still higher than that at control side, showing a significant difference between them (P < 0. 05). Conclusions Persistent over-expression of MAFbx and MuRF1 mRNA after muscle transfer has a close relationship with muscle atrophy and muscle dysfunction. MAFbx and MuRFI can be used as markers for early muscle atrophy, and also as potential target for drug treatment of muscle atrophy.