CAJ | 학술논문

目的研究负载日本血吸虫谷胱甘肽S-转移酶(glutathione S-transferase,GST)和可溶性虫卵抗原(soluble egg antigen,SEA)的树突状细胞的表型. 方法骨髓来源的细胞经白介素-4(interleukin-4,IL-4)、粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating factor,GM-CSF)诱导培养,获得树突状细胞,体外经GSI、SEA抗原刺激.用异硫氰酸荧光素(fluorescein isothiocyanate,FITC)标记的抗GST单克隆抗体染色法检测GST的负载情况.流式细胞仪检测血吸虫抗原负载后树突状细胞表面CD40、CD80、CD86、CD11c分子的表达情况,并与脂多糖(lipopolysaccharide,LPS)、PBS刺激组作比较. 结果 GST负载后在荧光显微镜下可观察到抗GST的特异荧光,表明抗原已被细胞摄取.与LPS相比较,GST、SEA抗原负载后,树突状细胞表面分子CD40、CD86上调不显著,而更类似于PBS刺激组. 结论日本血吸虫抗原负载后,树突状细胞的表型类似于未成熟表型.
목적 연구부재일본혈흡충곡광감태S-전이매(glutathione S-transferase,GST)화가용성충란항원(soluble egg antigen,SEA)적수돌상세포적표형. 방법 골수래원적세포경백개소-4(interleukin-4,IL-4)、립세포-거서세포집락자격인자(granulocyte-macrophage colony stimulating factor,GM-CSF)유도배양,획득수돌상세포,체외경GSI、SEA항원자격.용이류청산형광소(fluorescein isothiocyanate,FITC)표기적항GST단극륭항체염색법검측GST적부재정황.류식세포의검측혈흡충항원부재후수돌상세포표면CD40、CD80、CD86、CD11c분자적표체정황,병여지다당(lipopolysaccharide,LPS)、PBS자격조작비교. 결과 GST부재후재형광현미경하가관찰도항GST적특이형광,표명항원이피세포섭취.여LPS상비교,GST、SEA항원부재후,수돌상세포표면분자CD40、CD86상조불현저,이경유사우PBS자격조. 결론 일본혈흡충항원부재후,수돌상세포적표형유사우미성숙표형.
Objective To investigate the phenotype of dendritic cell( DC) loaded with GST and SEA from Schistosoma japonicum. Methods Bone marrow cells were cultured in the presence of IL-4 and GM-CSF to induce dendritic cells(DCs). These DCs were stimulated by purified GST and SEA antigen from Schistosoma japonicum, respectively. FITC labeled anti-GST monoclonal antibody was used to detect the loading of antigen. After that, the expression of CD40, CD80, CD86 and CD11c on the membrane of DC were analyzed by fluorescence activated cell sorting(FACS). Results The loading of antigen was confirmed by the detection of GST on the cells. GST was successfully loaded on DCs. The positive rates of CD40, CD80 and CD86 in the groups stimulated with GST or SEA were not raised significantly, comparing to the PBS control. Conclusion DC loaded with GST and SEA antigen from Schistosoma japonicum exhibited an immature phenotype.