CAJ | 학술논문

采用高脂饲料喂养并经高胰岛素-正常血糖钳夹技术评估,建立胰岛素抵抗大鼠模型.与正常饮食组相比,高脂饮食组大鼠血清游离脂肪酸和基础胰岛素水平升高(P＜0.05),平均葡萄糖输注率(GIR60-120)降低(P＜0.01),肝脏组织p-IRE-1α蛋白表达升高(P＜0.05).并且肝脏组织p-IRE-lα蛋白表达与血清游离脂肪酸水平呈正相关(r=0.627,P＜0.05).提示高脂饲料喂养可能通过升高血清游离脂肪酸水平,引起肝脏内质网应激,并通过p-IRE-1 α参与胰岛素抵抗的发生.
채용고지사료위양병경고이도소-정상혈당겸협기술평고,건립이도소저항대서모형.여정상음식조상비,고지음식조대서혈청유리지방산화기출이도소수평승고(P＜0.05),평균포도당수주솔(GIR60-120)강저(P＜0.01),간장조직p-IRE-1α단백표체승고(P＜0.05).병차간장조직p-IRE-lα단백표체여혈청유리지방산수평정정상관(r=0.627,P＜0.05).제시고지사료위양가능통과승고혈청유리지방산수평,인기간장내질망응격,병통과p-IRE-1 α삼여이도소저항적발생.
Fed with high-fat diet and assessed by hyperinsulinemia-euglycemia clamp technique, rat models with insulin resistance were successfully induced. Compared with normal chow group ( NC ), serum concentrations of free-fatty acids(FFAs) and baseline insulin in high-fat diet group(HF) was higher( P＜0.05 ), the average glucose infusion rate from 60 to 120 min( GIR60-120 ) was lower( P＜0.01 ), and the expression of p-IRE-lα in the liver was higher( P＜0.05 ). Furthermore, the expression of p-IRE-1α in the liver was positvely correlated with the serum concentration of FFAs. All these data indicate that high-fat diet may induce endoplasmic reticulum stress in the liver by elevating serum concentration of FFAs, and may participate in the genesis of insulin resistance via p-IRE-1α.