CAJ | 학술논문

目的验证下调miR-93表达对人脑胶质瘤细胞株的生长和侵袭抑制作用.方法合成miR-93抑制物,应用脂质体转染人脑胶质瘤细胞以抑制其表达,实时定量PCR检测转染后miR-93表达情况,流式细胞术检测、四甲基偶氮噻唑法(MTT)试验和软琼脂克隆形成实验评价细胞生长变化,tran-swell实验检测细胞侵袭能力的变化.结果转染miR-93抑制物后,细胞中miR-93高表达被明显抑制,细胞周期进展迟滞,S期细胞减少,生长速度减慢,克隆形成能力减弱,穿过matrigel胶的细胞数减少,侵袭能力被抑制.结论下调miR-93表达可抑制胶质瘤细胞的生长和侵袭.
목적 험증하조miR-93표체대인뇌효질류세포주적생장화침습억제작용.방법 합성miR-93억제물,응용지질체전염인뇌효질류세포이억제기표체,실시정량PCR검측전염후miR-93표체정황,류식세포술검측、사갑기우담새서법(MTT)시험화연경지극륭형성실험평개세포생장변화,tran-swell실험검측세포침습능력적변화.결과 전염miR-93억제물후,세포중miR-93고표체피명현억제,세포주기진전지체,S기세포감소,생장속도감만,극륭형성능력감약,천과matrigel효적세포수감소,침습능력피억제.결론 하조miR-93표체가억제효질류세포적생장화침습.
Objective To confirm the effect of miR-93 inhibitor in glioma cell growth and invasion.Methods Malignant glioma cells were transfected with miR-93 inhibitor by lipofectamin to downregulate their overexpression of miR-93.Real time-PCR was taken to measure miR-93 expression after transfection.The cell cycle kinetics and cell growth rate were detected by flowcytometry and MTT assay,the cell proliferative ability was evaluated by soft agar assay,and the invasive ability was detected by transwell assay.Results The highlevel expression of miR-93 was downregulated effectively in glioma cells after transfecting the miR-93 inhibitor.Meanwhile,the cell cycle progress was delayed,S phase cells were reduced,the speed of growth was slowed,cloning formation ability was receded,the number of cells through the matrigel was reduced,and invasive ability was significantly repressed.Conclusion Downregulation of miR-93 expression could inhibit the proliferative ability and invasive ability of glioma cells.