CAJ | 학술논문

目的研究外源性多巴胺对多巴胺能神经元的毒性作用。方法采用体外培养的PC12细胞为模型,以终浓度0.45 mmol/L的多巴胺对细胞进行诱导。结果在透射电镜下可见培养的PC12细胞核染色质明显固缩、断裂,原位末端标记技术显示胞核内散在分布断裂DNA片段,流式细胞仪检测到DNA周期中G0-G1期前出现明显的亚二倍体峰,琼脂糖凝胶电泳呈现典型的"梯状"带等细胞凋亡的特征性改变。结论外源性DA可诱导PC12细胞凋亡。
목적연구외원성다파알대다파알능신경원적독성작용。방법채용체외배양적PC12세포위모형,이종농도0.45 mmol/L적다파알대세포진행유도。결과재투사전경하가견배양적PC12세포핵염색질명현고축、단렬,원위말단표기기술현시포핵내산재분포단렬DNA편단,류식세포의검측도DNA주기중G0-G1기전출현명현적아이배체봉,경지당응효전영정현전형적"제상"대등세포조망적특정성개변。결론외원성DA가유도PC12세포조망。
Objective To study the toxicity of exogenous dopamine(DA) in dopaminergic neurons. Methods The PC12 cell was used as a cell model. Apoptosis was induced by DA. Results After addition 0.45 mmol/L DA, the PC12 cell showed condensed nucleus and chromatin under transmission electric microscope or detected by TUNEL. Meanwhile, there was a marked subdiploid peak before G0-G1 on DNA cycle with flow cytometry, and a very clear DNA ladder could be detected by gel electrophoresis. Conclusion The exogenous dopamine can induce apoptosis in PC12 cells.