中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2011年
4期
221-223
,共3页
范子锋%尹秀珍%王甲汉%吴永恒%李志清%易朝辉
範子鋒%尹秀珍%王甲漢%吳永恆%李誌清%易朝輝
범자봉%윤수진%왕갑한%오영항%리지청%역조휘
烧伤%海水浸泡%肿瘤坏死因子%白介素-6%创面愈合
燒傷%海水浸泡%腫瘤壞死因子%白介素-6%創麵愈閤
소상%해수침포%종류배사인자%백개소-6%창면유합
Burn%Seawater immersion%TNF- t%IL-6%Wound healing
目的 从创面炎症反应及感染等方面探讨海水浸泡对烫伤大鼠创面愈合的影响。方法 建立Wistar大鼠10％体表面积深Ⅱ度烫伤合并海水浸泡模型，采用数字表法，将120只雄性Wistar大鼠随机分为单纯烧伤对照组和海水浸泡实验组，实验组大鼠烫伤后立即用海水浸泡4h。观察组织病理改变、创面愈合时间和创面感染情况；ELISA法检测血清中TNF-α及IL-6含量。结果 实验组大鼠血清中TNF-α[( 130.4±17.3) pg/ml]、IL-6[(320.5±28.5) pg/ml]含量与对照组比较，差异有统计学意义(P＜0.05)；创面感染加重，创面愈合时间延长(P＜0.05)，再上皮化和表皮各层的分化延迟，且海水浸泡可使烧伤创面肿胀、创面局部组织炎症反应加重。结论 大鼠烫伤后合并海水浸泡，可加重创面炎症反应及创面感染，抑制创面愈合。
目的 從創麵炎癥反應及感染等方麵探討海水浸泡對燙傷大鼠創麵愈閤的影響。方法 建立Wistar大鼠10％體錶麵積深Ⅱ度燙傷閤併海水浸泡模型，採用數字錶法，將120隻雄性Wistar大鼠隨機分為單純燒傷對照組和海水浸泡實驗組，實驗組大鼠燙傷後立即用海水浸泡4h。觀察組織病理改變、創麵愈閤時間和創麵感染情況；ELISA法檢測血清中TNF-α及IL-6含量。結果 實驗組大鼠血清中TNF-α[( 130.4±17.3) pg/ml]、IL-6[(320.5±28.5) pg/ml]含量與對照組比較，差異有統計學意義(P＜0.05)；創麵感染加重，創麵愈閤時間延長(P＜0.05)，再上皮化和錶皮各層的分化延遲，且海水浸泡可使燒傷創麵腫脹、創麵跼部組織炎癥反應加重。結論 大鼠燙傷後閤併海水浸泡，可加重創麵炎癥反應及創麵感染，抑製創麵愈閤。
목적 종창면염증반응급감염등방면탐토해수침포대탕상대서창면유합적영향。방법 건립Wistar대서10％체표면적심Ⅱ도탕상합병해수침포모형，채용수자표법，장120지웅성Wistar대서수궤분위단순소상대조조화해수침포실험조，실험조대서탕상후립즉용해수침포4h。관찰조직병리개변、창면유합시간화창면감염정황；ELISA법검측혈청중TNF-α급IL-6함량。결과 실험조대서혈청중TNF-α[( 130.4±17.3) pg/ml]、IL-6[(320.5±28.5) pg/ml]함량여대조조비교，차이유통계학의의(P＜0.05)；창면감염가중，창면유합시간연장(P＜0.05)，재상피화화표피각층적분화연지，차해수침포가사소상창면종창、창면국부조직염증반응가중。결론 대서탕상후합병해수침포，가가중창면염증반응급창면감염，억제창면유합。
Objective To investigate the effects of seawater immersion on wound inflammation and healing following Ⅱ -degree scald in rats. Methods One hundred and twenty male Wistar rats with 10％ total body surface Ⅱ -degree scald were randomly divided into the scald group (group A) and the scald plus seawater immersion group ( group B). Rats in group B were immersed in seawater for 4 hours, soon after they were scalded. Wound infection, the time of healing and changes in histopathology were closely observed. Levels of serum interleukin- 6 ( IL- 6 ) and tumor necrosis factor ( TNF- α ) were determined with enzyme-linked immunosorbent assay (ELISA). Results Levels of serum IL- 6 (320. 5 ± 28.5 ) pg/ml and TNF-α content ( 130.4 ± 17.3 ) pg/ml in group B increased significantly, when they were compared with those of group A (P ＜0. 05 ). Moreover, wound surface was seriously infected and wound healing time prolonged (P ＜ 0. 05 ). Biological culture showed that seawater immersion could promote bacteria growth. Pathological examination indicated that seawater immersion could aggravate wound swelling and inflammatory response of the injured tissue, and inhibit epithelialization and differentiation of various epidermal layers. Conclusions Scald coupled with seawater immersion could aggravate inflammatory response of the wound surface and inhibit wound healing in rats.
