中国医学科学杂志(英文版)
中國醫學科學雜誌(英文版)
중국의학과학잡지(영문판)
CHINESE MEDICAL SCIENCES JOURNAL
2004年
1期
31-37
,共7页
贾延军%任会明%高新%季守平%杨军%刘泽鹏%李素波%章扬培
賈延軍%任會明%高新%季守平%楊軍%劉澤鵬%李素波%章颺培
가연군%임회명%고신%계수평%양군%류택붕%리소파%장양배
α-galactosidase gene%hyperacute rejection%Galα1,3Gal antigen
Objective To explore the strategies which reduce the amount of xenoantigen Galα1, 3 Gal. Methods Human α-galactosidase gene and α 1,2-fucosyltransferase gene were transferred into cultured porcine vascular endothelial cells PEDSV.15 and human α-galactosidase transgenic mice were produced. The Galα 1,3Gal on the cell surface and susceptibility of cells to human antibody-mediated lysis were analyzed. Results Human α-galactosidase gene alone reduced 78% of Galα1,3Gal on PEDSV.15 cell surface while human α-galactosidase combined with α 1,2-fucosyltransferase genes removed Galα 1,3Gal completely. Decrease of Galα1,3Gal could reduce susceptibility of cells to human antibody-mediated lysis, especially during co-expression of α-galactosidase gene and α1,2-fucosyltransferase gene. RT-PCR indicated positive human α-galactosidase gene expression in all organs of positive human α-galactosidase transgenic F1 mice including heart, liver, kidney, lung, and spleen, the amount of Galα1,3Gal antigens on which was reduced largely. 58% of spleen cells from F1 mice were destroyed by complement-mediated lysis compared with 24% of those from normal mice. Conclusions Human α-galactosidase gene and α1,2-fucosyltransferase gene effectively reduce the expression of Galα1,3Gal antigens on endothelial cell surface and confers resistance to human serummediated cytolysis. The expression of human α-galactosidase in mice can also eliminate the Galα1,3Gal antigens in most tissues and decrease the susceptibility of spleen cells to human serum-mediated cytolysis.