CAJ | 학술논문

目的评价多位点MLVA的15位点组合在北京基因型结核分枝杆菌(MTB)基因分型研究中的应用价值.方法采用MLVA(15位点)对来源于北京胸科医院的72株北京基因型MTB菌株进行基因分型,并将结果与金标准IS6110-RFLP的分型结果进行比较.结果 MLVA(15位点)分型后共得到59个类型,其中53个为独特类型,其总体分辨力为0.990,多态性较好的位点有Qub-11b、Mtub 21和QUB-26;IS6110-RFLP分型后共得到69个类型,其中66个为独特类型,分辨力高达0.999,并可对MLVA(15位点)中成簇的菌株进一步区分.结论 MLVA(15位点)在北京基因型菌株中具有较好的分辨能力,但成簇菌株仍需采用金标准IS6110-RFLP进一步细分.
목적 평개다위점MLVA적15위점조합재북경기인형결핵분지간균(MTB)기인분형연구중적응용개치.방법 채용MLVA(15위점)대래원우북경흉과의원적72주북경기인형MTB균주진행기인분형,병장결과여금표준IS6110-RFLP적분형결과진행비교.결과 MLVA(15위점)분형후공득도59개류형,기중53개위독특류형,기총체분변력위0.990,다태성교호적위점유Qub-11b、Mtub 21화QUB-26;IS6110-RFLP분형후공득도69개류형,기중66개위독특류형,분변력고체0.999,병가대MLVA(15위점)중성족적균주진일보구분.결론 MLVA(15위점)재북경기인형균주중구유교호적분변능력,단성족균주잉수채용금표준IS6110-RFLP진일보세분.
Objective To evaluate the application of multiple locus variable number tandem repeat analysis (MLVA, 15-locus set)for genotyping of Mycobacterium tuberculosis (MTB) strains of Beijing genotype. Methods Total 72 Beijing genotype MTB strains obtained from Beijing Thoracic Hospital were genotyped by MLVA (15-locus set). The results were compared with that generated from "gold standard"IS6110-RFLP. Results After genotyped by MLVA ( 15-locus set), 72 strains were grouped into 59 types,of which 53 were unique types. The Hunter-Gaston index (HGI) of MLVA ( 15-locus set) was 0.990. The loci QUB-11b, Mtub 21 and QUB-26 were polymorphic in selected Beijing genotype strains. Genotyping by IS6110-RFLP generated 69 types, of which 66 were unique types. The HGI of IS6110-RFLP was up to 0.999, and the MLVA (15-locus set) clustered strains could be further subdivided. Conclusion MLVA(15-locus set) showed better discriminatory ability in Beijing genotype MTB strains, though secondary typing of clustered strains by IS6110-RFLP is needed.