中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2009年
5期
416-419
,共4页
宋卫青%李化会%陈华波%原江水%殷晓婕
宋衛青%李化會%陳華波%原江水%慇曉婕
송위청%리화회%진화파%원강수%은효첩
系统性红斑狼疮%干扰素调节因子5%单核苷酸多态性
繫統性紅斑狼瘡%榦擾素調節因子5%單覈苷痠多態性
계통성홍반랑창%간우소조절인자5%단핵감산다태성
Systemic lupus erythernatosus%IRF5%Single nueleotide polymorphism
目的 研究山东地区汉族人群干扰素调节因子5(IRF5)基因rs2004640、rs10954213单核苷酸多态性,探讨其与系统性红斑狼疮(SLE)易感性之间的关系.方法 采用聚合酶链反应和限制性片段长度多态性等方法对92例SLE患者和88名健康对照IRF5基因rs2004640 G/T、rs10954213 G/A多态性进行分析,计算基因型和等位基因频率.结果 SLE患者IRF5 rs2004640GG、GT、TT基因型频率分别是0.198、0.521和0.281,与对照组比较差异有统计学意义(X2=8.73,P<0.05);SLE患者IRF5 rs10954213 GG、GA、从基因型频率分别是0.318、0.409和0.273,与对照组间差异有统计学意义(X2=6.36,P<0.05).结论 山东汉族人群IRF5基因位点rs2004640、rs10954213的多态性,可能与山东地区汉族人群SLE的易感性有关,需进一步累积更多数据证实.
目的 研究山東地區漢族人群榦擾素調節因子5(IRF5)基因rs2004640、rs10954213單覈苷痠多態性,探討其與繫統性紅斑狼瘡(SLE)易感性之間的關繫.方法 採用聚閤酶鏈反應和限製性片段長度多態性等方法對92例SLE患者和88名健康對照IRF5基因rs2004640 G/T、rs10954213 G/A多態性進行分析,計算基因型和等位基因頻率.結果 SLE患者IRF5 rs2004640GG、GT、TT基因型頻率分彆是0.198、0.521和0.281,與對照組比較差異有統計學意義(X2=8.73,P<0.05);SLE患者IRF5 rs10954213 GG、GA、從基因型頻率分彆是0.318、0.409和0.273,與對照組間差異有統計學意義(X2=6.36,P<0.05).結論 山東漢族人群IRF5基因位點rs2004640、rs10954213的多態性,可能與山東地區漢族人群SLE的易感性有關,需進一步纍積更多數據證實.
목적 연구산동지구한족인군간우소조절인자5(IRF5)기인rs2004640、rs10954213단핵감산다태성,탐토기여계통성홍반랑창(SLE)역감성지간적관계.방법 채용취합매련반응화한제성편단장도다태성등방법대92례SLE환자화88명건강대조IRF5기인rs2004640 G/T、rs10954213 G/A다태성진행분석,계산기인형화등위기인빈솔.결과 SLE환자IRF5 rs2004640GG、GT、TT기인형빈솔분별시0.198、0.521화0.281,여대조조비교차이유통계학의의(X2=8.73,P<0.05);SLE환자IRF5 rs10954213 GG、GA、종기인형빈솔분별시0.318、0.409화0.273,여대조조간차이유통계학의의(X2=6.36,P<0.05).결론 산동한족인군IRF5기인위점rs2004640、rs10954213적다태성,가능여산동지구한족인군SLE적역감성유관,수진일보루적경다수거증실.
Objective To investigate two single nucleotide polymorphism sites of IRF5 and to de-tect their relationship with SLE in a population from Shandong province. Methods The polymorphisms (rs2004640 G/T,rs10954213 G/A) were detected with PCR-RFLP in 92 eases of SLE and 88 healthy con-trols. The genotype and allele frequencies were calculated and analyzed. Results The genotype frequencies Of GG, GT and TT in rs2004640 site in SLE were 0. 198, 0.521 and 0.281, respectively. The difference was significant between SLE and centrol (X2 = 8.73, P < 0.05). The genotype frequencies of GG, GA and AA in rs10954213 site in SLE were 0. 318, 0. 409 and 0.273, respectively. The differenee was significant between SLE and control (X2 = 6. 36, P < 0. 05). Conclusion The polymorphism of rs2004640, rs10954213 in IRF5 may be associated with SLE in the population of Han nationality from Shandong province of China.