CAJ | 학술논문

目的观察蓝莓对大鼠肝纤维化及肝细胞超微结构的影响.方法 60只健康清洁级Wistar大鼠随机分为正常对照组(A组)、肝纤维化模型组(B组)、蓝莓原浆低、中、高剂量组(C、D、E组)及复方鳖甲软肝片组(F组),共6组,每组10只.除正常对照组外,其余各组均腹腔注射猪血清制备大鼠肝纤维化模型,C、D、E、F组在造模同时分别给予蓝莓原浆或复方鳖甲软肝片灌胃,1次/d,共12周.12周末处死大鼠,行肝脏HE、Masson染色,并在透射电镜下观察肝组织超微结构变化.计算大鼠肝脏指数、测定血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平、肝组织匀浆超氧化物歧化酶(SOD)活性、丙二醛(MDA)、羟脯氨酸(Hyp)及还原型谷胱甘肽(GSH)含量.结果各组大鼠血清ALT、AST水平差异无统计学意义(P＞0.05)；蓝莓原浆中、高剂量组肝组织匀浆SOD活性、GSH含量明显高于肝纤维化模型组(均P＜0.05),肝脏指数、MDA及Hyp含量明显低于肝纤维化模型组(均P＜0.05)；肝纤维化程度明显减轻(P＜0.05),胶原表达减少,超微结构病变明显改善.结论蓝莓对猪血清所致大鼠肝纤维化有一定的预防作用,对肝细胞线粒体等细胞器损伤有一定的保护作用,以蓝莓原浆中、高剂量组效果较好.其机制可能与其增加肝脏SOD活性及GSH含量,降低MDA含量,提高机体抗氧化应激的能力有关.
목적 관찰람매대대서간섬유화급간세포초미결구적영향.방법 60지건강청길급Wistar대서수궤분위정상대조조(A조)、간섬유화모형조(B조)、람매원장저、중、고제량조(C、D、E조)급복방별갑연간편조(F조),공6조,매조10지.제정상대조조외,기여각조균복강주사저혈청제비대서간섬유화모형,C、D、E、F조재조모동시분별급여람매원장혹복방별갑연간편관위,1차/d,공12주.12주말처사대서,행간장HE、Masson염색,병재투사전경하관찰간조직초미결구변화.계산대서간장지수、측정혈청병안산전안매(ALT)、천동안산전안매(AST)수평、간조직균장초양화물기화매(SOD)활성、병이철(MDA)、간포안산(Hyp)급환원형곡광감태(GSH)함량.결과 각조대서혈청ALT、AST수평차이무통계학의의(P＞0.05)；람매원장중、고제량조간조직균장SOD활성、GSH함량명현고우간섬유화모형조(균P＜0.05),간장지수、MDA급Hyp함량명현저우간섬유화모형조(균P＜0.05)；간섬유화정도명현감경(P＜0.05),효원표체감소,초미결구병변명현개선.결론람매대저혈청소치대서간섬유화유일정적예방작용,대간세포선립체등세포기손상유일정적보호작용,이람매원장중、고제량조효과교호.기궤제가능여기증가간장SOD활성급GSH함량,강저MDA함량,제고궤체항양화응격적능력유관.
Objective To observe the effects of blueberry on rat with hepatic fibrosis and ultrastructural.of hepatocytes.Methods Sixty (60) healthy Wistar rats were randomly divided into six groups:normal control group( group A),hepatic fibrosis model group( group B),blueberry at low,middle and high concentration groups ( group C,D,E),Fu-Fang-Bie-Jia-Ruan-Gan tablet group ( group F).The hepatic fibrosis model of rat was established by intraperitoneal injection of porcine serum once daily for 12 weeks.Simultaneously,rats in groups C - F were respectively perfused with blueberry juice or Fu-Fang-BieJia-Ruan-Gan tablet for 12 weeks except for the normal control group which accepted saline alone.Upon terminal sacrifices of all rats at the end of the twelve weeks.Pathology of hepatic tissue was evaluated by hematoxylin-eosin (HE), Masson staining and transmission electron microscope. Liver index were measured.Levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were examined.Activities of superoxide dismutase (SOD),the contents of malondialdehyde (MDA),hydroxyproline (Hyp) and reduced glutathione (GSH) in liver homogenates were determined.Results Refer to the serum levels of ALT and AST,there is no significant difference existed in all groups(P ＞ 0.05).Activities of SOD,the contents of GSH in liver homogenates of group D and E were significantly higher than those of group B ( P ＜ 0.05 ),while liver index,the contents of Hyp,MDA in liver homogenates were significantly lower than those of group B ( P ＜ 0.05 ).Compared with the group B,the pathological stages of hepatic fibrosis in group D and E were significantly reduced ( P ＜ 0.05 ),the expression of collagen were decreased,ultrastructural alterations were markedly improved. Conclusion Blueberry may have certain preventional protective effects on porcine serum induced rat hepatic fibrosis and exhibit certain protective effects on organelles of hepatocytes ( such as mitochondria),especially in middle and high does of blueberry.Its mechanism is probably related to increase the ability of antioxidant stress by increasing SOD activity and GSH content and reducing MDA content.