南京中医药大学学报
南京中醫藥大學學報
남경중의약대학학보
JOURNAL OF NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2010年
1期
39-41
,共3页
江励华%黄臻%段金廒%王明艳
江勵華%黃臻%段金廒%王明豔
강려화%황진%단금오%왕명염
华佗健聪汤%星形胶质细胞%脑源性神经生长因子%氧化应激
華佗健聰湯%星形膠質細胞%腦源性神經生長因子%氧化應激
화타건총탕%성형효질세포%뇌원성신경생장인자%양화응격
Huatuo Jiancong Decoction%Astrocytes%brain-derivedneurotrophic factor( BDNF)%oxidative stress
目的 观察华佗健聪汤对氧化应激状态下的星形胶质细胞分泌脑源性神经生长因子(BDNF)的影响.方法 以原代培养的sD乳鼠星形胶质细胞为材料,H_2O_2造成星形胶质细胞损伤,氧化损伤前中药组用华佗健聪汤灌胃后获取的大鼠含药血清进行干预,用ELISA法检测星形胶质细胞上清液中BDNF含量.结果 中药组BDNF含量与正常组、模型组相比均有显著增加(P<0.05,P<0.01).结论 华佗健聪汤能明显增加在氧化应激状态下星形胶质细胞分泌BDNF的水平,从而保护神经元.
目的 觀察華佗健聰湯對氧化應激狀態下的星形膠質細胞分泌腦源性神經生長因子(BDNF)的影響.方法 以原代培養的sD乳鼠星形膠質細胞為材料,H_2O_2造成星形膠質細胞損傷,氧化損傷前中藥組用華佗健聰湯灌胃後穫取的大鼠含藥血清進行榦預,用ELISA法檢測星形膠質細胞上清液中BDNF含量.結果 中藥組BDNF含量與正常組、模型組相比均有顯著增加(P<0.05,P<0.01).結論 華佗健聰湯能明顯增加在氧化應激狀態下星形膠質細胞分泌BDNF的水平,從而保護神經元.
목적 관찰화타건총탕대양화응격상태하적성형효질세포분비뇌원성신경생장인자(BDNF)적영향.방법 이원대배양적sD유서성형효질세포위재료,H_2O_2조성성형효질세포손상,양화손상전중약조용화타건총탕관위후획취적대서함약혈청진행간예,용ELISA법검측성형효질세포상청액중BDNF함량.결과 중약조BDNF함량여정상조、모형조상비균유현저증가(P<0.05,P<0.01).결론 화타건총탕능명현증가재양화응격상태하성형효질세포분비BDNF적수평,종이보호신경원.
OBJECTIVE To study the effect of Huatuo Jiancong Decotion on levels of brain-derived neurotrophic factor(BDNF) excreted from primary cultured astrocytes under the state of oxidative stress. METHOD The primary cultured murine astrocytes are injured by H_2O_2.In adance the Chinese medicine group was given the serum of the rats containing medicineingredients, which the rats assimilated the medicine ingredients from Huatuo Jiancong Decoction Infused intragastricly into the rats.The levels of BDNF of astrocytes are examined by KLISA. RESULT The level of BDNF is greatly more increasing in the Chinese medicine group than the normal and model groups. CONCLUSION Huatuo Jiancong Decoction greatly improves the level of BDNF of primary cultured astrocytes under the state of oxidative stress and then protects nerve cells.
