CAJ | 학술논문

为了探明长江中上游流域黄颡鱼野生群体的遗传多样性状况和遗传结构,本研究采用磁珠富集法筛选出10个黄颡鱼微卫星标记,并利用其对长江中上游流域3个黄颡鱼(Pelteobagrus fulvidrac)野生群体(赤水群体、乐山群体、洞庭群体)的遗传结构进行分析.获得65个阳性克隆并测序,得到36个含有微卫星的序列,设计并合成了22对微卫星引物,经筛选得到10对多态性稳定的引物,其中高多态性位点6个,中多态位点3个.每对引物等位基因数2-9个,平均4.8.3个群体(赤水、乐山和洞庭)的平均多态信息含量(PIC)分别为0 5438、0.4568和0.3965,平均有效等位基因(N_e)分别为2.9928、2.5401和2.1713,平均期望杂合度(H_e)分别为0.6280、0.5277和0.4757,表明这3个群体遗传多样性水平较高,其中赤水群体遗传多样性最高,洞庭群体最低.种群分化指数(F_(st))和遗传距离(D_s)分析表明,洞庭群体和乐山群体之间的亲缘关系最近,而与赤水群体的亲缘关系最远.聚类分析显示,乐山群体和洞庭群体聚为一支,赤水群体单独聚为一支.
위료탐명장강중상유류역황상어야생군체적유전다양성상황화유전결구,본연구채용자주부집법사선출10개황상어미위성표기,병이용기대장강중상유류역3개황상어(Pelteobagrus fulvidrac)야생군체(적수군체、악산군체、동정군체)적유전결구진행분석.획득65개양성극륭병측서,득도36개함유미위성적서렬,설계병합성료22대미위성인물,경사선득도10대다태성은정적인물,기중고다태성위점6개,중다태위점3개.매대인물등위기인수2-9개,평균4.8.3개군체(적수、악산화동정)적평균다태신식함량(PIC)분별위0 5438、0.4568화0.3965,평균유효등위기인(N_e)분별위2.9928、2.5401화2.1713,평균기망잡합도(H_e)분별위0.6280、0.5277화0.4757,표명저3개군체유전다양성수평교고,기중적수군체유전다양성최고,동정군체최저.충군분화지수(F_(st))화유전거리(D_s)분석표명,동정군체화악산군체지간적친연관계최근,이여적수군체적친연관계최원.취류분석현시,악산군체화동정군체취위일지,적수군체단독취위일지.
In order to explore the genetic diversity and genetic structure of yellow catfish(Pelteobagrus fulvidraco)in different areas along the middle-upper reaches of the Yangtze River,we had developed ten polymorphic microsatellite markers using FIASCO(Fast Isolation by AFLP of Sequences containing repeats)magnetic beads method,and used them to evaluate genetic variation in three geographic populations of yellow catfish(Chishui Leshan and Dongting)along the middle-upper reaches of the Yangtze River 65 positive clones were selected and sequenced,of which 36 clones were discovered to contain microsatellite repeats 22 pairs of SSR-primers were synthesized,and 10 polymorphic microsatellite markers were screened out,six of them were high polymorphic loci and three of them were middle The mean polymorphism information content(PCI)of Chishui,Leshan and Dongting population was 0 .5438,0.4568 and 0.3965,respectively; Mean effective number of alleles(N_e) was 2.9928,2.5401 and 2.1713,respectively; Mean expected heterozygosity(H_e)was 0.6280,0.5277 and 0. 4757,respectively Results showed that all three populations had abundant genetic diversity,and Chishui population was the highest among them,Dongting population was the poorest Population differentiation values(F_(st))and genetic distance(D_s)analysis showed that Leshan population were genetically closer to Dongting population,and the relationship between these two populations and Chishui population were farther UPGMA cluster analysis based on genetic distance indicated that Leshan and Dongting populations were in one cluster,and Chishui population was another cluster.