CAJ | 학술논문

目的探讨鼻咽癌患者血小板源性生长因子-BB(PDGF-BB)的表达水平及其临床意义. 方法采用酶联免疫吸附试验(ELISA)检测45例鼻咽癌患者和20例正常对照者外周血血清中PDGF-BB的水平,用半定量反转录聚合酶链反应(RT-PCR)技术检测受试者外周血单个核细胞(PBMCs)中PDGF-B mRNA的表达水平. 结果鼻咽癌患者和正常对照组血清PDGF-BB水平分别为(401±74)、(28±8)ng/L,外周血单个核细胞PDGF-B mRNA的表达水平分别为(0.835±0.078)、(0.228±0.038)相对灰度值,差异均有统计学的意义(P<0.05). 结论 PDGF-BB在鼻咽癌的发生发展中可能起着重要作用,检测血清PDGF-BB及PBMCs中PDGF-B mRNA表达水平可能对鼻咽癌诊断具有一定临床指导意义.
목적 탐토비인암환자혈소판원성생장인자-BB(PDGF-BB)적표체수평급기림상의의. 방법 채용매련면역흡부시험(ELISA)검측45례비인암환자화20례정상대조자외주혈혈청중PDGF-BB적수평,용반정량반전록취합매련반응(RT-PCR)기술검측수시자외주혈단개핵세포(PBMCs)중PDGF-B mRNA적표체수평. 결과 비인암환자화정상대조조혈청PDGF-BB수평분별위(401±74)、(28±8)ng/L,외주혈단개핵세포PDGF-B mRNA적표체수평분별위(0.835±0.078)、(0.228±0.038)상대회도치,차이균유통계학적의의(P<0.05). 결론 PDGF-BB재비인암적발생발전중가능기착중요작용,검측혈청PDGF-BB급PBMCs중PDGF-B mRNA표체수평가능대비인암진단구유일정림상지도의의.
Objective To investigate the expression of platelet-derived growth factor-BB(PDGF-BB) and its role in the pathogenesis of nasopharyngeal carcinoma.Methods The levels of serum PDGF-BB were determined by enzyme linked immunosorbent assay (ELISA) and the level of PDGF-B mRNA in peripheral blood mononuclear cells by reverse transcription polymerase chain reaction (RT-PCR) in 45 nasopharyngeal carcinoma patients and 20 normal controls.Results The levels of PDGF-BB and PDGF-B mRNA in peripheral blood mononuclear cells were significantly higher in patients with nasopharyngeal carcinoma(401±74 ng/L,0.835±0.078 ng/L,respectively)than in controls(28±8 ng/L,0.228±0.038 ng/L,respectively)(P<0.05)..Conclusion PDGF-BB may play an important role in the onset and development of nasopharyngeal carcinoma.The levels of PDGF-BB,PDGF-B mRNA may be of some clinical significance in nasopharyngeal carcinoma diagnoses.