中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2012年
6期
630-633
,共4页
溃疡性结肠炎%肠易激综合征%树突状细胞%白细胞介素23%免疫组化
潰瘍性結腸炎%腸易激綜閤徵%樹突狀細胞%白細胞介素23%免疫組化
궤양성결장염%장역격종합정%수돌상세포%백세포개소23%면역조화
Ulcerative colitis%Irritable bowel syndrome%Dendritic cell%Interleukin-23%Immunohistochemistry
目的 探讨溃疡性结肠炎(UC)与肠易激综合征(IBS)患者肠黏膜组织中5100阳性表达的树突状细胞(S100+DC)、CD83阳性表达的树突状细胞(CD83+DC)与白细胞介素(IL) 23P19的表达差异.方法 以60例UC、60例IBS患者的结肠镜活检组织蜡块标本为研究对象,应用免疫组化SP法检测两种病变肠黏膜组织中S100+ DC、CD83+DC及IL-23 P19的表达情况.结果 两种病变组织中S100+DC、CD83+ DC阳性表达率差异无统计学意义(P＞0.05)；IL-23P19阳性表达率差异有统计学意义(85.0％与70.0％,x2=3.87,P＜0.05)；轻型UC组S100+ DC、CD83+DC及IL-23P19三个指标阳性细胞浸润密度分别为( 24.43±8.26)、(5.23±1.66)、(95.03±18.39)个/mm2,重型UC组分别为(36.13±11.36)、(10.13±3.29)、( 133.38±25.32)个/mm2,两组对应的指标比较差异均有统计学意义(P均＜0.05)；全结肠型与局部结肠型UC的三个指标阳性细胞浸润密度比较,差异均无统计学意义(P均＞0.05).结论 UC组织中DC的浸润密度增高且分化能力增强,IL-23P19分泌增多；UC组织中,随着病情的加重S100+ DC、CD83+ DC、IL-23P19阳性表达明显增多.
目的 探討潰瘍性結腸炎(UC)與腸易激綜閤徵(IBS)患者腸黏膜組織中5100暘性錶達的樹突狀細胞(S100+DC)、CD83暘性錶達的樹突狀細胞(CD83+DC)與白細胞介素(IL) 23P19的錶達差異.方法 以60例UC、60例IBS患者的結腸鏡活檢組織蠟塊標本為研究對象,應用免疫組化SP法檢測兩種病變腸黏膜組織中S100+ DC、CD83+DC及IL-23 P19的錶達情況.結果 兩種病變組織中S100+DC、CD83+ DC暘性錶達率差異無統計學意義(P＞0.05)；IL-23P19暘性錶達率差異有統計學意義(85.0％與70.0％,x2=3.87,P＜0.05)；輕型UC組S100+ DC、CD83+DC及IL-23P19三箇指標暘性細胞浸潤密度分彆為( 24.43±8.26)、(5.23±1.66)、(95.03±18.39)箇/mm2,重型UC組分彆為(36.13±11.36)、(10.13±3.29)、( 133.38±25.32)箇/mm2,兩組對應的指標比較差異均有統計學意義(P均＜0.05)；全結腸型與跼部結腸型UC的三箇指標暘性細胞浸潤密度比較,差異均無統計學意義(P均＞0.05).結論 UC組織中DC的浸潤密度增高且分化能力增彊,IL-23P19分泌增多；UC組織中,隨著病情的加重S100+ DC、CD83+ DC、IL-23P19暘性錶達明顯增多.
목적 탐토궤양성결장염(UC)여장역격종합정(IBS)환자장점막조직중5100양성표체적수돌상세포(S100+DC)、CD83양성표체적수돌상세포(CD83+DC)여백세포개소(IL) 23P19적표체차이.방법 이60례UC、60례IBS환자적결장경활검조직사괴표본위연구대상,응용면역조화SP법검측량충병변장점막조직중S100+ DC、CD83+DC급IL-23 P19적표체정황.결과 량충병변조직중S100+DC、CD83+ DC양성표체솔차이무통계학의의(P＞0.05)；IL-23P19양성표체솔차이유통계학의의(85.0％여70.0％,x2=3.87,P＜0.05)；경형UC조S100+ DC、CD83+DC급IL-23P19삼개지표양성세포침윤밀도분별위( 24.43±8.26)、(5.23±1.66)、(95.03±18.39)개/mm2,중형UC조분별위(36.13±11.36)、(10.13±3.29)、( 133.38±25.32)개/mm2,량조대응적지표비교차이균유통계학의의(P균＜0.05)；전결장형여국부결장형UC적삼개지표양성세포침윤밀도비교,차이균무통계학의의(P균＞0.05).결론 UC조직중DC적침윤밀도증고차분화능력증강,IL-23P19분비증다；UC조직중,수착병정적가중S100+ DC、CD83+ DC、IL-23P19양성표체명현증다.
Objective To compare the levels of S100+ DC,CD83 + DC and IL-23P19 between the patients with mucosa of ulcerative colitis ( UC ) and those with irritable bowel syndrome ( 0BS ).Methods Paraffin-embedded colon tissue from colonoscopic biopsy was detected in sixty UC and sixty IBS cases for the positivity of SI00 + DC,CD83 + DC and IL-23P19 using immunohistochemical SP method.Results There was no significant difference in the positive rates of S100 + DC and CD83 + DC between the two groups ( P ＞ 0.05 ),while significant difference in IL-23P19 level was found between the two groups( 85.0％ vs.70.0％ ; x2 =3.87,P ＜0.05 ).The infiltration densities of S100 + DC,CD83 + DC and expression of IL-23 P19 in the mild and severe UC patients were 24.43 ± 8.26/mm2,5.23 ± 1.66/mm2,95.03 ± 18.39/mm2,and 36.13 ± 11.36/mm2,10.13 ±3.29/mm2,133.38 ± 25.32/mm2,respectively.The difference between the mild and severe UC patients was significant ( P ＜ 0.05 ).No significant difference was found regarding the above three parameters between severity-stratified subgroups of the UC patients ( P ＞ 0.05 ).Conclusion In UC colon tissues,the infiltration density and activity of DC were increased.IL-23P19 level was elevated.The levels of S100 + DC,CD83 + DC and IL-23P19 were increased with the advancement of UC disease.
