CAJ | 학술논문

目的探讨幻肢痛大鼠脊髓背角小胶质细胞和星形胶质细胞数量的变化.方法健康成年SD大鼠11只,雌雄不拘,体重290～300 g,采用随机数字表法,将其随机分为2组:假手术组(S组,n=5)和单侧坐骨神经横断组(SNT组,n=6).术后持续观察SNT组大鼠自噬情况,并进行自噬评分.术后28d时取L5节段脊髓组织,分别进行iba-1(标记小胶质细胞)及胶质纤维酸性蛋白(标记星形胶质细胞)免疫组化染色,进行手术侧和非手术侧脊髓背角小胶质细胞和星形胶质细胞的计数.结果 S组无一只大鼠发生自噬,SNT组术后2d开始陆续发生自噬,最高自噬评分9～11分.与S组比较,SNT组手术侧脊髓背角小胶质细胞和星形胶质细胞数量均减少(P＜0.05).结论幻肢痛大鼠脊髓背角小胶质细胞和星形胶质细胞数量减少.
목적 탐토환지통대서척수배각소효질세포화성형효질세포수량적변화.방법 건강성년SD대서11지,자웅불구,체중290～300 g,채용수궤수자표법,장기수궤분위2조:가수술조(S조,n=5)화단측좌골신경횡단조(SNT조,n=6).술후지속관찰SNT조대서자서정황,병진행자서평분.술후28d시취L5절단척수조직,분별진행iba-1(표기소효질세포)급효질섬유산성단백(표기성형효질세포)면역조화염색,진행수술측화비수술측척수배각소효질세포화성형효질세포적계수.결과 S조무일지대서발생자서,SNT조술후2d개시륙속발생자서,최고자서평분9～11분.여S조비교,SNT조수술측척수배각소효질세포화성형효질세포수량균감소(P＜0.05).결론 환지통대서척수배각소효질세포화성형효질세포수량감소.
Objective To investigate the changes in the number of microglias and astrocytes in the spinal dorsal born in a rat model of phantom limb pain.Methods Eleven healthy adult SD rats of both sexes weighing 290-300 g were randomly divided into 2 groups:sham operation group (group S,n =5 ) and unilateral sciatic nerve transection group (group SNT,n =6).Phantom limb pain model was induced by resection of a 0.5 cm segment of unilateral sciatic nerve in group SNT.In group S unilateral sciatic nerve was exposed but not transected.The animals were observed for autotomy and scored (0 =no autotomy,13 =the worst autotomy) after operation and were sacrificed on the 28th day after operation.The L5 segment of the spinal cord was removed for determination of the number of microglials (by iba-1 immuno-histochemistry) and astrocytes (by GFAP immuno-histochemistry).Results In group S no animal developed autotomy.In group SNT autotomy started from the 2nd day after operation and the score reached 9-11.Compared with group S,the number of the microglias and astrocytes in the spinal dorsal horn was significantly decreased in the operated side in group SNT ( P ＜ 0.05 ).Conclusion The number of microglias and astrocytes in the spinal dorsal horn is decreased in animals with phantom limb pain.