CAJ | 학술논문

目的观察大鼠胸腺内注射异基因抗原在同种异体异基因股静脉移植免疫耐受中的作用.方法将48只SD大鼠随机分为4组:自体股静脉移植组(A组)、异体股静脉移植组(B组)、异体股静脉移植免疫抑制剂组(C组)、胸腺内注射供体组织相容性(MHC)抗原后移植组(D组).于2周后进行影像学、组织学、免疫学检测.结果组织学检测结果显示:D组、C组急性排斥反应损伤较轻,B组血管壁的各层结构破坏最重,可见大量炎性细胞浸润.B组受体大鼠血清干扰素(IFN)-γ浓度为(86.707±10.928)ng/L,显著高于A、C、D组[(29.328±4.170)、(69.076±8.059)、(63.355±4.895)ng/L,P<0.05];B组受体大鼠血清白细胞介素(IL)-4浓度为(23.656±3.369)ng/L,显著低于C、D组[(29.425±4.174)、(31.000±4.659)ng/L,P<0.05].结论胸腺内注射异基因MHC抗原可诱导大鼠对同种异体血管移植的特异性免疫耐受.
목적 관찰대서흉선내주사이기인항원재동충이체이기인고정맥이식면역내수중적작용.방법 장48지SD대서수궤분위4조:자체고정맥이식조(A조)、이체고정맥이식조(B조)、이체고정맥이식면역억제제조(C조)、흉선내주사공체조직상용성(MHC)항원후이식조(D조).우2주후진행영상학、조직학、면역학검측.결과 조직학검측결과현시:D조、C조급성배척반응손상교경,B조혈관벽적각층결구파배최중,가견대량염성세포침윤.B조수체대서혈청간우소(IFN)-γ농도위(86.707±10.928)ng/L,현저고우A、C、D조[(29.328±4.170)、(69.076±8.059)、(63.355±4.895)ng/L,P<0.05];B조수체대서혈청백세포개소(IL)-4농도위(23.656±3.369)ng/L,현저저우C、D조[(29.425±4.174)、(31.000±4.659)ng/L,P<0.05].결론 흉선내주사이기인MHC항원가유도대서대동충이체혈관이식적특이성면역내수.
Objective To investigate the possibility of inducing immune tolerance to vein trans-plantation in rats by intrathymic injection of allogene. Methods MHC antigen extracted from splenic cells of donor Wistar rats was intrathymically injected to recipients SD rats, and donor Wistar femur vein was transplanted after 2 weeks. Forty-eight SD rats were randomly divided into 4 groups: group A ( femur vein autograft) ,group B ( femur vein allograft) ,group C ( femur vein allograft with use of immunosuppressant), group D (intrathymus injection). Imaging, histology and immunological assays of these groups were carried out 2 weeks after the transplantation. Results Histologic parameters that were tested were better in group D than those in group B. The concentration of serum IFN-γ in group B was significantly higher than that in groups A, C and D [ ( 86. 707±10.928 ) ng/L vs (29.328±4. 170), (69.076±8.059) and (63.355± 4.895) ng/L respectively, P < 0.05 ]. The concentration of serum IL-4 in group B was obviously lower than in groups C and D [ (23.656±3.369) ng/L vs (29.425±4.174) and ( 31.000±4.659) ng/L re-spectively,P < 0.05 ]. Conclusion Intrathymus injection of allogenic antigen might induce specific im-mune tolerance to femur vein transplantation in rats.