CAJ | 학술논문

目的探讨IFN-γ对多发性骨髓瘤(multiple myeloma,MM)细胞上B细胞活化因子受体(BAFF-R)的表达变化影响及相关机制.方法应用RT-PCR和ELISA法检测IFN-γ诱导多发性骨髓瘤细胞BAFF-R的表达水平,观察NF-κB特异性抑制剂BAY11-7082对其mRNA和蛋白表达水平的影响;应用Western blot检测NF-κB在IFN-γ诱导的多发性骨髓瘤细胞反应中的活化程度.结果 IFN-γ以时间和剂量依赖方式上调多发性骨髓瘤细胞BAFF-R表达.BAY11-7082以剂量依赖方式在基因转录和翻译水平显著抑制IFN-γ诱导的BAFF-R表达.结论 NF-κB信号通路参与了多发性骨髓瘤细胞BAFF-R的表达,如何参与有待进一步研究.
목적 탐토IFN-γ대다발성골수류(multiple myeloma,MM)세포상B세포활화인자수체(BAFF-R)적표체변화영향급상관궤제.방법 응용RT-PCR화ELISA법검측IFN-γ유도다발성골수류세포BAFF-R적표체수평,관찰NF-κB특이성억제제BAY11-7082대기mRNA화단백표체수평적영향;응용Western blot검측NF-κB재IFN-γ유도적다발성골수류세포반응중적활화정도.결과 IFN-γ이시간화제량의뢰방식상조다발성골수류세포BAFF-R표체.BAY11-7082이제량의뢰방식재기인전록화번역수평현저억제IFN-γ유도적BAFF-R표체.결론 NF-κB신호통로삼여료다발성골수류세포BAFF-R적표체,여하삼여유대진일보연구.
Objective To investigate the action and mechanism of NF-κB pathway in up-regulating B cell-activating factor receptor (BAFF-R) expression in multiple myeloma cells induced by IFN-γ.Methods Activated NF-κB were detected with Western blot, while the expression of BAFF-R were measured with RT-PCR and ELISA, and investigated the effect of BAY11-7082 on transcription of BAFF-R mRNA and translation of protein in multiple myeloma cells stimulated by IFN-γ. Results IFN-γ can induce the degradation of IκB-α in time-dependent and dosage-dependent manner, and up-regulated BAFF-R expression in multiple myeloma cells. BAY11-7082, an NF-κB inhibitor, inhibited not only the transcription of BAFF-R mRNA but also the protein of regulated by IFN-γin dosage-dependent manner. Conclusion NFκB may play an important role in high expression of BAFF-R in multiple myeloma cells induced by IFN-γ.