CAJ | 학술논문

目的观察红细胞生成素(EPO)对补体成分C3片段C3a介导的肾小管上皮细胞间充质转分化(EMT)的影响.方法将人近端肾小管上皮细胞(HK-2)分为6组:对照组、EPO组、TGF-β组、TGF-β+EPO组、C3a组、EPO+C3a组,分别用RT-PCR、Western印迹和细胞免疫荧光方法检测HK-2细胞α-SMA、E-cadherin、C3的mRNA和蛋白表达.结果与对照组和EPO组比较,C3a或TGF-β干预HK2细胞后,αt-SMA mRNA和蛋白表达增强(均P＜0.05),E-cadherin mRNA和蛋白表达减少(均P＜ 0.05),补体C3 mRNA和蛋白表达增强(均P＜ 0.05)；而同时给予EPO干预后,C3a或TGF-β的上述作用可被明显减弱(均P＜0.05).结论 EPO可抑制C3a介导的肾小管上皮细胞EMT.
목적 관찰홍세포생성소(EPO)대보체성분C3편단C3a개도적신소관상피세포간충질전분화(EMT)적영향.방법 장인근단신소관상피세포(HK-2)분위6조:대조조、EPO조、TGF-β조、TGF-β+EPO조、C3a조、EPO+C3a조,분별용RT-PCR、Western인적화세포면역형광방법검측HK-2세포α-SMA、E-cadherin、C3적mRNA화단백표체.결과 여대조조화EPO조비교,C3a혹TGF-β간예HK2세포후,αt-SMA mRNA화단백표체증강(균P＜0.05),E-cadherin mRNA화단백표체감소(균P＜ 0.05),보체C3 mRNA화단백표체증강(균P＜ 0.05)；이동시급여EPO간예후,C3a혹TGF-β적상술작용가피명현감약(균P＜0.05).결론 EPO가억제C3a개도적신소관상피세포EMT.
Objective To investigate the effects of erythropoietin (EPO) on complement 3a (C3a)-induced renal tubular epithelial to mesenchymal transition. Methods The HK-2 cells were divided into 6 groups namely control group,EPO group,TGF-β group,TGF-β+EPO group,C3a group and EPO+C3a group.The mRNA and protein expressions of α-SMA,E-cadherin and C3 were investigated by RT-PCR,Western blot and immunofluorescence respectively. Results Compared with control group and EPO group,the mRNA and protein expressions of α-SMA in HK-2 cells were up-regulated after the intervention of C3a or TGF-β (all P＜0.05).On the contrast,the mRNA and protein expressions of E-cadherin were down-regulated(P＜0.05),the mRNA and protein expressions of C3 were enhanced (all P＜0.05).However,all those above effects of C3a or TGF-β were inhibited after the intervention of EPO (all P＜0.05). Conclusion EPO is capable of suppressing the epithelial to mesenchymal transition induced by C3a.