中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2010年
7期
690-692
,共3页
X线照射%脊髓损伤%运动功能
X線照射%脊髓損傷%運動功能
X선조사%척수손상%운동공능
X-irradiation%Spinal cord injury%Locomotor function
目的 探讨脊髓挫伤部位的X线照射治疗对脊髓损伤后运动功能恢复的作用.方法 将70只雌性Wistar大鼠按照纽约大学的重力冲击方法建立大鼠脊髓(T10)损伤动物模型,按照随机数字表法分为7组,每组10只,其中6组大鼠在损伤后不同时间(损伤后20 min、1 d、2 d、4 d、7 d、17 d)对挫伤部位进行X线(20 Gy)照射,第7组则不予照射(对照组).然后根据Basso、Beattie、Bresnahan(BBB)评分标准评测各组大鼠运动功能恢复情况,并进行统计学比较.采用快蓝染色对存活6周以上的大鼠进行挫伤脊髓的组织形态学观察.结果脊髓挫伤后20 min、1 d、2 d行X线照射组BBB评分明显高于对照组,差异均有统计学意义(P<0.05),且在脊髓损伤后的2～3周进展较快,后期恢复缓慢.组织形态学观察可见应用X线治疗组周边组织残存区面积大于对照组.结论 脊髓挫伤部位伤后早期行X线照射治疗可保护脊髓残存的神经组织,改善运动功能的恢复.
目的 探討脊髓挫傷部位的X線照射治療對脊髓損傷後運動功能恢複的作用.方法 將70隻雌性Wistar大鼠按照紐約大學的重力遲擊方法建立大鼠脊髓(T10)損傷動物模型,按照隨機數字錶法分為7組,每組10隻,其中6組大鼠在損傷後不同時間(損傷後20 min、1 d、2 d、4 d、7 d、17 d)對挫傷部位進行X線(20 Gy)照射,第7組則不予照射(對照組).然後根據Basso、Beattie、Bresnahan(BBB)評分標準評測各組大鼠運動功能恢複情況,併進行統計學比較.採用快藍染色對存活6週以上的大鼠進行挫傷脊髓的組織形態學觀察.結果脊髓挫傷後20 min、1 d、2 d行X線照射組BBB評分明顯高于對照組,差異均有統計學意義(P<0.05),且在脊髓損傷後的2～3週進展較快,後期恢複緩慢.組織形態學觀察可見應用X線治療組週邊組織殘存區麵積大于對照組.結論 脊髓挫傷部位傷後早期行X線照射治療可保護脊髓殘存的神經組織,改善運動功能的恢複.
목적 탐토척수좌상부위적X선조사치료대척수손상후운동공능회복적작용.방법 장70지자성Wistar대서안조뉴약대학적중력충격방법건립대서척수(T10)손상동물모형,안조수궤수자표법분위7조,매조10지,기중6조대서재손상후불동시간(손상후20 min、1 d、2 d、4 d、7 d、17 d)대좌상부위진행X선(20 Gy)조사,제7조칙불여조사(대조조).연후근거Basso、Beattie、Bresnahan(BBB)평분표준평측각조대서운동공능회복정황,병진행통계학비교.채용쾌람염색대존활6주이상적대서진행좌상척수적조직형태학관찰.결과척수좌상후20 min、1 d、2 d행X선조사조BBB평분명현고우대조조,차이균유통계학의의(P<0.05),차재척수손상후적2～3주진전교쾌,후기회복완만.조직형태학관찰가견응용X선치료조주변조직잔존구면적대우대조조.결론 척수좌상부위상후조기행X선조사치료가보호척수잔존적신경조직,개선운동공능적회복.
Objective To determine whether the X-irradiation of the contusion site can improve the recovery of locomotor function following spinal cord injury in rats. Methods The spinal cords of 70 male Wistar rats at the level of T10 were contused with a weight-drop apparatus similar to the NYU impactor. Ten rats were chosen as control group. The other 60 rats were equally randomized into 6 groups and localized X-irradiation (20 Gy) of the contusion site was performed on rats in these 6 group 20 min, 1,2,4, 7 and 17 d after the injury, respectively. Locomotor function was determined by the Basso, Beattie and Bresnahan Locomotor Rating Scale (BBB). Following 6 w of behavioral evaluation, the spinal cords were received histological examination. Results X-irradiation of the contusion site 20 min, 1 and 2 d after the injury significantly improved the recovery of locomotor function as compared with no X-irradiation (control group, P<0.05); and the improvement in the 2nd and 3rd w were the quickest among all the time points. Increased amount of spared spinal cord tissue in the X-irradiation groups was found as compared with that in the control group. Conclusion X-irradiation of the contusion site following contusion at an early stage can protect the spared spinal cord tissue and help the recovery of locomotor function.
