CAJ | 학술논문

背景:基质细胞衍生因子是嗜淋巴细胞人类免疫缺陷病毒1感染的体外潜在抑制因子,可阻断人类免疫缺陷病毒1侵入人体的通路.目的:了解人类免疫缺陷病毒1感染相关等位基因基质细胞衍生因子-3'A在我国南方畲族人群中的频率和多态性分布,探讨畲族人未感染人类免疫缺陷病毒1的基因水平的可能原因.设计:单一样本研究.单位:赣南医学院生化与分子生物学教研室.对象:于1995-01/12从畲族主要居住地江西省铅山县、福建省宁德地区和浙江省景宁畲族自治县随机选择3代均为畲族的186名无人类免疫缺陷病毒1感染的无关个体作为观察对象.方法:随机采集血样186份,提取基因组DNA.经PCR-RFLP分析,计算基因突变频率;并对群体分布和性别分布进行统计分析.主要观察指标:中国畲族人群基质细胞衍生因子1基因型构成.结果:186人的数据均进入结果分析,无脱落者.基质细胞衍生因子-3'A频率为19.6%,群体分布符合Hardy-Weinberg平衡,性别之间无差异.结论:南方畲族人的基质细胞衍生因子-3'A基因突变可能延缓艾滋病的发病进程,这一发现对于我国南方畲族人的艾滋病防治具有积极意义.
배경:기질세포연생인자시기림파세포인류면역결함병독1감염적체외잠재억제인자,가조단인류면역결함병독1침입인체적통로.목적:료해인류면역결함병독1감염상관등위기인기질세포연생인자-3'A재아국남방여족인군중적빈솔화다태성분포,탐토여족인미감염인류면역결함병독1적기인수평적가능원인.설계:단일양본연구.단위:공남의학원생화여분자생물학교연실.대상:우1995-01/12종여족주요거주지강서성연산현、복건성저덕지구화절강성경저여족자치현수궤선택3대균위여족적186명무인류면역결함병독1감염적무관개체작위관찰대상.방법:수궤채집혈양186빈,제취기인조DNA.경PCR-RFLP분석,계산기인돌변빈솔;병대군체분포화성별분포진행통계분석.주요관찰지표:중국여족인군기질세포연생인자1기인형구성.결과:186인적수거균진입결과분석,무탈락자.기질세포연생인자-3'A빈솔위19.6%,군체분포부합Hardy-Weinberg평형,성별지간무차이.결론:남방여족인적기질세포연생인자-3'A기인돌변가능연완애자병적발병진정,저일발현대우아국남방여족인적애자병방치구유적겁의의.
BACKGROUND: Stromal cell-derived factor 1 (SDF1), a potential inhibitor of infection by lymphophilic HIV-1 strains, can help to block the pathway of HIV-1 invasion into the human body.OBJECTIVE: Genotype and polymorphism of SDF1-3 'A allele associated with HIV-1 infection were investigated in She Ethnic Group in the south of China so as explore the possible causes of uninfection by HIV-1 strains among this population.DESIGN: Single sample study.SETTING: Department of Biochemistry and Molecular Biology, Gannan Medical College.PARTICIPANTS: Totally 186 She Ethnic subjects without HIV-1 infection collected randomly from those whose three generations belonged to She Ethnic Group, and inhabited in Qianshan County of Jiangxi Province,Ningde area of Fujian Province and Jingning She County of Zhejiang Province, from January to December 1995.METHODS: The whole blood samples from 186 She Ethnic subjects were collected randomly, and then their genomic DNA samples were extracted respectively. Allelic polymorphism was examined by the polymerase chain reaction and restriction-fragment-length polymorphisms (PCR-RFLP).MAIN OUTCOME MEASURES: The distribution of SDF1-3'A allele in She Ethnic Group in the south of China.RESULTS: The data of 186 She Ethnic subjects entered the result analysis without any loss in the midway. The frequency of SDF1-3 'A allele in She Ethnic Group samples was 19.6%, and the allelic distribution of the gene was in accordance with Hardy-Weinberg equilibrium. No difference was found between male and female individuals.CONCLUSION: The frequency of SDF1-3 'A allele of She Ethnic Group in the south of China was similar to that of Dai Nationality in Yunnan.Based on its slow-down effect on clinical course of AIDS, the mutation of SDF1-3'A is significant in the prevention and treatment of AIDS in She Ethnic Group in the south of China.