CAJ | 학술논문

目的研究血管内皮细胞生长因子(VEGF)基因多态性与糖尿病肾病(DN)的关系.方法单纯2型糖尿病(DM)组76例,DN组81例,健康对照(NC)组60例.UNIQ-10柱提取全血基因组DNA.标本基因型的判断用聚合酶链反应-限制性酶切片断长度多态性技术.Hardy-Weinberg平衡法检验各组基因频率的群体代表性.结果 (1)DN组VEGF-460和+405 CC基因型频率和C等位基因频率明显高于DM组和NC组.(2)-460位点CC基因型DN患病率明显高于CT和TT基因型.+405位点CC基因型DN患病率明显高于CG和GG基因型.(3)显示VEGF-460和+405基因多态性均为DN发生的独屯危险因素.结论 (1)VEGF-460C/T基因多态性与DN发生有关.C等位基因可能是DN易感基因.(2)VEGF+405G/C基因多态性与DN发生有关.C等位基因可能是DN的易感基因.
목적 연구혈관내피세포생장인자(VEGF)기인다태성여당뇨병신병(DN)적관계.방법 단순2형당뇨병(DM)조76례,DN조81례,건강대조(NC)조60례.UNIQ-10주제취전혈기인조DNA.표본기인형적판단용취합매련반응-한제성매절편단장도다태성기술.Hardy-Weinberg평형법검험각조기인빈솔적군체대표성.결과 (1)DN조VEGF-460화+405 CC기인형빈솔화C등위기인빈솔명현고우DM조화NC조.(2)-460위점CC기인형DN환병솔명현고우CT화TT기인형.+405위점CC기인형DN환병솔명현고우CG화GG기인형.(3)현시VEGF-460화+405기인다태성균위DN발생적독둔위험인소.결론 (1)VEGF-460C/T기인다태성여DN발생유관.C등위기인가능시DN역감기인.(2)VEGF+405G/C기인다태성여DN발생유관.C등위기인가능시DN적역감기인.
Objective To study the relationship between polymorphisms of vascular endothelial growth factor (VEGF) gene and diabetic nephropathy. Methods Genotyping for the VEGF gene -460 and +405 polymorphisms was carried out by PCR-RFLP analysis. Chi-square analysis was used to test VEGF genotype distribution followed by Hardy-Weinberg equilibrium. Results (1)VEGF-460 and +405 genotype CC and allele C frequencies were significantly increased in patients with DN versus without DN and controls. (2)Morbidity rate of DN was significantly increased in VEGF-460 CC genotype compared with CT and TT genotype. Morbidity rate of DN was higher in VEGF+405 CC genotype than in CG and GG genotype. (3)Logistic regression analysis revealed that the VEGF-460C/T and +405C/G polymorphism were independent risk factors for DN. Conclusions (1)VEGF-460C/T polymorphism is associated with DN and C allele probably is a susceptible gene for type 2 diabetic nephropathy. (2)VEGF-460 C/G polymorphism is associated with DN and C allele probably is a susceptible gene for type 2 diabetic nephropathy