中华口腔医学研究杂志（电子版）
中華口腔醫學研究雜誌（電子版）
중화구강의학연구잡지（전자판）
CHINESE JOURNAL OF STOMATOLOGICAL RESEARCH(ELECTRONIC VERSION)
2009年
4期
361-368
,共8页
武东辉%李劲松%林钊宇%陈伟良%潘朝斌%廖文婷%俞春萍%孔庆丽%宋立兵
武東輝%李勁鬆%林釗宇%陳偉良%潘朝斌%廖文婷%俞春萍%孔慶麗%宋立兵
무동휘%리경송%림쇠우%진위량%반조빈%료문정%유춘평%공경려%송립병
舌鳞状细胞癌%着丝粒蛋白-H%增殖
舌鱗狀細胞癌%著絲粒蛋白-H%增殖
설린상세포암%착사립단백-H%증식
Squamous cell carcinoma of the tongue%CENP-H%Proliferation
目的 探讨导致恶性肿瘤出现细胞染色体非整倍数现象的着丝粒蛋白-H(CENP-H)在舌鳞癌中的表达情况,及其对舌鳞癌细胞增殖的影响.方法 舌鳞状细胞癌组织及其癌旁舌黏膜组织组成配对子,采用RT-PCR、Western blot分别检测8对配对子的舌鳞癌系TSCCa、Tca8113及原代培养的舌黏膜细胞中CENP-H mRNA水平和蛋白的表达水平;采用免疫组织化学SP法检测同样8对配对子舌黏膜中CENP-H的表达;采用MTT法、克隆形成实验法分析转染CENP-H对TSCCa细胞增殖能力的影响.结果 舌鳞状细胞癌中CENP-H在mRNA、蛋白质水平的表达高于舌黏膜上皮,且差异有统计学意义;免疫组化显示CENP-H高表达,且阳性染色定位于肿瘤细胞的细胞核,而在舌黏膜鳞状上皮细胞中未见表达;MTT法、克隆形成试验显示转染CENP-H后舌鳞癌细胞增殖能力明显高于未转染的对照组细胞(P ＜ 0.001).结论 舌鳞癌细胞中高表达的CENP-H对舌鳞癌细胞增殖起到促进作用,在舌鳞癌的发生发展中可能扮演重要角色.
目的 探討導緻噁性腫瘤齣現細胞染色體非整倍數現象的著絲粒蛋白-H(CENP-H)在舌鱗癌中的錶達情況,及其對舌鱗癌細胞增殖的影響.方法 舌鱗狀細胞癌組織及其癌徬舌黏膜組織組成配對子,採用RT-PCR、Western blot分彆檢測8對配對子的舌鱗癌繫TSCCa、Tca8113及原代培養的舌黏膜細胞中CENP-H mRNA水平和蛋白的錶達水平;採用免疫組織化學SP法檢測同樣8對配對子舌黏膜中CENP-H的錶達;採用MTT法、剋隆形成實驗法分析轉染CENP-H對TSCCa細胞增殖能力的影響.結果 舌鱗狀細胞癌中CENP-H在mRNA、蛋白質水平的錶達高于舌黏膜上皮,且差異有統計學意義;免疫組化顯示CENP-H高錶達,且暘性染色定位于腫瘤細胞的細胞覈,而在舌黏膜鱗狀上皮細胞中未見錶達;MTT法、剋隆形成試驗顯示轉染CENP-H後舌鱗癌細胞增殖能力明顯高于未轉染的對照組細胞(P ＜ 0.001).結論 舌鱗癌細胞中高錶達的CENP-H對舌鱗癌細胞增殖起到促進作用,在舌鱗癌的髮生髮展中可能扮縯重要角色.
목적 탐토도치악성종류출현세포염색체비정배수현상적착사립단백-H(CENP-H)재설린암중적표체정황,급기대설린암세포증식적영향.방법 설린상세포암조직급기암방설점막조직조성배대자,채용RT-PCR、Western blot분별검측8대배대자적설린암계TSCCa、Tca8113급원대배양적설점막세포중CENP-H mRNA수평화단백적표체수평;채용면역조직화학SP법검측동양8대배대자설점막중CENP-H적표체;채용MTT법、극륭형성실험법분석전염CENP-H대TSCCa세포증식능력적영향.결과 설린상세포암중CENP-H재mRNA、단백질수평적표체고우설점막상피,차차이유통계학의의;면역조화현시CENP-H고표체,차양성염색정위우종류세포적세포핵,이재설점막린상상피세포중미견표체;MTT법、극륭형성시험현시전염CENP-H후설린암세포증식능력명현고우미전염적대조조세포(P ＜ 0.001).결론 설린암세포중고표체적CENP-H대설린암세포증식기도촉진작용,재설린암적발생발전중가능분연중요각색.
Objective To investigate the expression pattern and promoting proliferation function of centromere protein H (CENP-H) in Squamous cell carcinoma of the tongue which has been demonstrated to be associated with aneuploidy in carcinoma cell. Methods Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot analysis were performed to test the expression of CENP-H in eight matched pairs of Squamous cell carcinoma of the tongue tissues (C) and adjacent noncancerous tongue tissues (N), as well as Squamous cell carcinoma of the tongue(TSCC) cell lines and tongue epithelial cells (TEC), the level of CENP-H in 8 characterized TSCC tissues was determined by immunohistochemistry. MTT assay, colony formation assay displayed that TSCCa with high expression of CENP-H reproductive activity. Results The mRNA and protein level of CENP-H was much higher in cancer tissues (C) and TSCC cell lines than that in adjacent noncancerous tongue tissues (N) and in normal cells, respectively. MTT assay, colony formation assay displayed that high expression of CENP-H promoted the proliferation of TSCC cell line TSCCa (P ＜ 0.001). Conclusion CENP-H is highly expressed in TSCC, and its high expression promotes proliferation.
