上海医学
上海醫學
상해의학
SHANGHAI MEDICAL JOURNAL
2009年
10期
892-895
,共4页
尖锐湿疣%人乳头瘤病毒%液相芯片
尖銳濕疣%人乳頭瘤病毒%液相芯片
첨예습우%인유두류병독%액상심편
Condyloma acuminata%Human papillomavirus%Liquid chip
目的 采用液相芯片技术分析上海地区尖锐湿疣患者人乳头瘤病毒(HPV)型别的分布情况.方法 从116例尖锐湿疣患者的疣体标本中提取脱氧核糖核酸(DNA),应用液相芯片技术进行HPV基因型检测,并与聚合酶链反应(PCR)的检测结果进行比较,明确液相芯片的检测效能.结果 液相芯片检测的116例标本中,HPV阳性114例,阳性率为98.3%.其中单一型别感染占71.6%(83/116),双重型别感染占18.1%(21/116),三重型别感染占7.8%(9/116),四重型别感染占0.9%(1/116).共检出18种HPV亚型,其中HPV6和HPVll最为常见,感染率分别为60.3%(70/116)和42.2%(49/116).在高危型HPV感染中,最常见的3种亚型依次为HPV16、HPV52和HPVl8.液相芯片与PCR检测结果的符合率为90.7%,并且液相芯片对多重感染的检出率高于PCR.结论 上海地区尖锐湿疣以HPV6和HPV11感染为主.高危型HPV感染中最常见的3种亚型依次为HPV16、HPV52和HPV18.液相芯片对HPV多重感染的检出率高于PCR.
目的 採用液相芯片技術分析上海地區尖銳濕疣患者人乳頭瘤病毒(HPV)型彆的分佈情況.方法 從116例尖銳濕疣患者的疣體標本中提取脫氧覈糖覈痠(DNA),應用液相芯片技術進行HPV基因型檢測,併與聚閤酶鏈反應(PCR)的檢測結果進行比較,明確液相芯片的檢測效能.結果 液相芯片檢測的116例標本中,HPV暘性114例,暘性率為98.3%.其中單一型彆感染佔71.6%(83/116),雙重型彆感染佔18.1%(21/116),三重型彆感染佔7.8%(9/116),四重型彆感染佔0.9%(1/116).共檢齣18種HPV亞型,其中HPV6和HPVll最為常見,感染率分彆為60.3%(70/116)和42.2%(49/116).在高危型HPV感染中,最常見的3種亞型依次為HPV16、HPV52和HPVl8.液相芯片與PCR檢測結果的符閤率為90.7%,併且液相芯片對多重感染的檢齣率高于PCR.結論 上海地區尖銳濕疣以HPV6和HPV11感染為主.高危型HPV感染中最常見的3種亞型依次為HPV16、HPV52和HPV18.液相芯片對HPV多重感染的檢齣率高于PCR.
목적 채용액상심편기술분석상해지구첨예습우환자인유두류병독(HPV)형별적분포정황.방법 종116례첨예습우환자적우체표본중제취탈양핵당핵산(DNA),응용액상심편기술진행HPV기인형검측,병여취합매련반응(PCR)적검측결과진행비교,명학액상심편적검측효능.결과 액상심편검측적116례표본중,HPV양성114례,양성솔위98.3%.기중단일형별감염점71.6%(83/116),쌍중형별감염점18.1%(21/116),삼중형별감염점7.8%(9/116),사중형별감염점0.9%(1/116).공검출18충HPV아형,기중HPV6화HPVll최위상견,감염솔분별위60.3%(70/116)화42.2%(49/116).재고위형HPV감염중,최상견적3충아형의차위HPV16、HPV52화HPVl8.액상심편여PCR검측결과적부합솔위90.7%,병차액상심편대다중감염적검출솔고우PCR.결론 상해지구첨예습우이HPV6화HPV11감염위주.고위형HPV감염중최상견적3충아형의차위HPV16、HPV52화HPV18.액상심편대HPV다중감염적검출솔고우PCR.
Objective To use liquid chips (luminex method) for investigating the distribution of human papillomavirus (HPV) genotypes in patients with condyloma acuminata in Shanghai. Methods The biopsy specimens were obtained from 116 patients with condyloma acuminate and the deoxyribonucleic acids were extracted for analysis using liquid chips; the results were compared with those obtained by type-specific polymerase chain reaction (PCR) so as to assess the efficacy of liquid chips. Results Liquid chips showed that 114 of the 116 specimens were HPV positive, with the positive rate being 98.3%, including 71.6% (83/116) with single infection, 18.1% (21/116) with double infection, 7.8% (9/116) with triple infection and 0.9% (1/116) with quadruple infection. Eighteen genotypes were identified by liquid chips, among which HPV 6 and HPV 11 were the two most common types, with infection rates being 60.3 % (70/116) and 42.2 % (49/116), respectively. The top three high-risk HPV genotypes were HPV16, HPV52 and HPV18. The consistent rate between liquid chips and PCR for HPV genotyping was 90.7%. Besides, liquid chip had a high sensitivity than PCR for detection of multiple infection. Conclusion HPV6 and HPVl 1 are the 2 major HPV genotypes in patients with condyloma acuminata in Shanghai. The top three high-risk HPV genotypes are HPV16,HPV52 and HPV18 in order. The liquid chips have a higher sensitivity than PCR for dectection of HPV.
