CAJ | 학술논문

背景:骨髓间充质干细胞系统性输注后,何种因素促使其迁移到正确部位尤为关键,目前认为黏附分子在介导骨髓间充质干细胞向缺血或损伤组织迁移过程中起重要作用.目的:观察血管细胞黏附分子1与细胞间黏附分子1在大鼠骨髓间充质干细胞中的表达.方法:采用直接贴壁法体外分离培养大鼠骨髓间充质干细胞,免疫细胞化学染色检测血管细胞黏附分子1及细胞间黏附分子1蛋白的表达,应用免疫荧光直标法在流式细胞仪上检测血管细胞黏附分子1及细胞间黏附分子1抗原的表达率,RT-PCR半定量分析血管细胞黏附分子1及细胞间黏附分子1 mRNA的表达.结果与结论:免疫细胞化学染色结果显示,骨髓间充质干细胞血管细胞黏附分子1呈弱阳性表达,细胞间黏附分子1呈强阳性表达.流式细胞仪检测结果显示,血管细胞黏附分子1表达率为6%,细胞间黏附分子1表达率为100%.RT-PCR检测结果显示,血管细胞黏附分子1 mRNA呈微弱表达,细胞间黏附分子1 mRNA呈高度表达.提示在生理状态下,体外培养的大鼠骨髓间充质干细胞低表达血管细胞黏附分子1,高表达细胞间黏附分子1.
배경:골수간충질간세포계통성수주후,하충인소촉사기천이도정학부위우위관건,목전인위점부분자재개도골수간충질간세포향결혈혹손상조직천이과정중기중요작용.목적:관찰혈관세포점부분자1여세포간점부분자1재대서골수간충질간세포중적표체.방법:채용직접첩벽법체외분리배양대서골수간충질간세포,면역세포화학염색검측혈관세포점부분자1급세포간점부분자1단백적표체,응용면역형광직표법재류식세포의상검측혈관세포점부분자1급세포간점부분자1항원적표체솔,RT-PCR반정량분석혈관세포점부분자1급세포간점부분자1 mRNA적표체.결과여결론:면역세포화학염색결과현시,골수간충질간세포혈관세포점부분자1정약양성표체,세포간점부분자1정강양성표체.류식세포의검측결과현시,혈관세포점부분자1표체솔위6%,세포간점부분자1표체솔위100%.RT-PCR검측결과현시,혈관세포점부분자1 mRNA정미약표체,세포간점부분자1 mRNA정고도표체.제시재생리상태하,체외배양적대서골수간충질간세포저표체혈관세포점부분자1,고표체세포간점부분자1.
BACKGROUND: Following bone marrow mesenchymal stem cells (BMMSCs) infusion therapy, which factor promotes BMMSCs migrated to correct position is a key point, currently, adhesion molecule is thought to be playing an important role in mediating BMMSCs migration. OBJECTIVE: To investigate the expression of vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) in rat BMMSCs. METHODS: BMMSCs were in vitro separated from rat bone marrow by directly adherence method. The expression of VCAM-1 and ICAM-1 were identified by using immunocytochemical staining, and the expression rates of antigen were tested by flow cytometry, in addition, their mRNA expressions were measured by RT-PCR. RESULTS AND CONCLUSION: Immunocytochemistry demonstrated that BMMSCs weakly expressed VCAM-1, but strong expressed ICAM-1. Flow cytometry showed that the expression rate of VCAM-1 was 6%, and the expression rate of ICAM-1 was 100%. RT-PCR showed that BMMSCs expressed a low level of VCAM-1 mRNA but a high level of ICAM-1 mRNA. It revealed under physiological condition, BMMSCs expressed a low level of VCAM-1, whereas they expressed a high level of ICAM-1.