生态环境学报
生態環境學報
생태배경학보
ECOLOGY AND ENVIRONMENT
2010年
2期
330-333
,共4页
张卫%林匡飞%张巍%龙亿涛%刘莉莉%王学东
張衛%林劻飛%張巍%龍億濤%劉莉莉%王學東
장위%림광비%장외%룡억도%류리리%왕학동
菲%土壤%微生物降解%优势菌
菲%土壤%微生物降解%優勢菌
비%토양%미생물강해%우세균
phenanthrene%soil%microbial degradation%dominant bacterium
研究了不同条件土壤中多环芳烃菲的降解动态.结果表明:温度和底物浓度对土壤中菲的降解有较大影响,未灭菌土壤中菲的降解半衰期为5.1 d;从污染土壤中分离到一株高效降解菲的菌株,经16S rDNA鉴定为产碱杆菌属(Alcaligenes bacterium LBM.),同源性高达99%;随着优势菌接种量增加,基础培养基中菲降解速率逐渐加快;Fe~(3+)、Co~(2+)和Cu~(2+)对优势菌降解菲能力均有不同程度影响,其中以Fe~(3+)影响最明显.
研究瞭不同條件土壤中多環芳烴菲的降解動態.結果錶明:溫度和底物濃度對土壤中菲的降解有較大影響,未滅菌土壤中菲的降解半衰期為5.1 d;從汙染土壤中分離到一株高效降解菲的菌株,經16S rDNA鑒定為產堿桿菌屬(Alcaligenes bacterium LBM.),同源性高達99%;隨著優勢菌接種量增加,基礎培養基中菲降解速率逐漸加快;Fe~(3+)、Co~(2+)和Cu~(2+)對優勢菌降解菲能力均有不同程度影響,其中以Fe~(3+)影響最明顯.
연구료불동조건토양중다배방경비적강해동태.결과표명:온도화저물농도대토양중비적강해유교대영향,미멸균토양중비적강해반쇠기위5.1 d;종오염토양중분리도일주고효강해비적균주,경16S rDNA감정위산감간균속(Alcaligenes bacterium LBM.),동원성고체99%;수착우세균접충량증가,기출배양기중비강해속솔축점가쾌;Fe~(3+)、Co~(2+)화Cu~(2+)대우세균강해비능력균유불동정도영향,기중이Fe~(3+)영향최명현.
The degradation kinetics of Phenanthrene were studied in different soils. The results showed that temperature and substrate concentration can obviously affect Phenanthrene degradation rate in soil, and the half-life of Phenanthrene degradation in the non-sterilized soil was only 5.1 d, which showed that microorganism played an important role. The dominant degradation bacterium which can effectively degrade Phenanthrene was screened from the tested soil sample and identified by 16S rDNA as Alcaligenes bacterium LBM. When the inoculation amount of dominant bacteria increased, the decomposition rate was accelerated. Fe~(3+), Co~(2+) and Cu~(2+) had some effects on the microbial degradation of Phenanthrene, and Fe~(3+) affected the rate most distinctly.