国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2010年
14期
1743-1746
,共4页
聚合酶链式反应%甘油三酯%HCV-RNA
聚閤酶鏈式反應%甘油三酯%HCV-RNA
취합매련식반응%감유삼지%HCV-RNA
Polymerase chain reaction%Triglyceride%HCV-RNA
目的 探讨脂血标本对丙肝病毒RNA定量检测结果 的影响程度.方法 在逆转录酶的作用下将DNA逆转录为HCV-cDNA,以四种脱氧核甘酸为底物,用Taqman探针法对HCV-RNA进行实时定量检测用于评价不同浓度的甘油三酯对低水平和高水平的HCV-RNA结果 的影响.结果 当标本为含104 IU/ml丙肝病毒,其甘油三酯浓度上升为4mmol/L病毒载量从104IU/ml降为103 IU/ml,检测结果 的差异有统计学意义;当标本为含107、106、103 IU/ml丙肝病毒,HCV-RNA的水平与甘油三酯的浓度没有明显关系.结论 脂血标本对低水平HCV-RNA的检测存在干扰,而对较高水平的HCV-RNA不存在明显的干扰.
目的 探討脂血標本對丙肝病毒RNA定量檢測結果 的影響程度.方法 在逆轉錄酶的作用下將DNA逆轉錄為HCV-cDNA,以四種脫氧覈甘痠為底物,用Taqman探針法對HCV-RNA進行實時定量檢測用于評價不同濃度的甘油三酯對低水平和高水平的HCV-RNA結果 的影響.結果 噹標本為含104 IU/ml丙肝病毒,其甘油三酯濃度上升為4mmol/L病毒載量從104IU/ml降為103 IU/ml,檢測結果 的差異有統計學意義;噹標本為含107、106、103 IU/ml丙肝病毒,HCV-RNA的水平與甘油三酯的濃度沒有明顯關繫.結論 脂血標本對低水平HCV-RNA的檢測存在榦擾,而對較高水平的HCV-RNA不存在明顯的榦擾.
목적 탐토지혈표본대병간병독RNA정량검측결과 적영향정도.방법 재역전록매적작용하장DNA역전록위HCV-cDNA,이사충탈양핵감산위저물,용Taqman탐침법대HCV-RNA진행실시정량검측용우평개불동농도적감유삼지대저수평화고수평적HCV-RNA결과 적영향.결과 당표본위함104 IU/ml병간병독,기감유삼지농도상승위4mmol/L병독재량종104IU/ml강위103 IU/ml,검측결과 적차이유통계학의의;당표본위함107、106、103 IU/ml병간병독,HCV-RNA적수평여감유삼지적농도몰유명현관계.결론 지혈표본대저수평HCV-RNA적검측존재간우,이대교고수평적HCV-RNA불존재명현적간우.
Objective To investigate the effect of blood samples rich in triglyceride on the results of quantitative test of hepatitis C virus RNA.Methods Under the action of reverse-transcriptase,DNA was reversely transcribed into HCV-cDNA.Using four types of deoxyribonucleotide as a substrate,HCV-RNA was detected by real-time fluorescent quantitative method with TaqMan probe to evaluate the effects of different concentrations of triglyceride on the test results of low- and high-load of HCV-RNA.Results In the sample containing viral load of 104 IU/ml,the viral load was declined from 104 IU/ml to 103 IU/ ml with an increase in triglyceride concentration to 4mmol/L,the test result had a statistical significance; while the viral load increased to 107,106 or 105 IU/ml,there was no marked relationship between the test result of HCV-RNA and the concentration of triglyceride.Conclusions Blood samples rich in triglyceride has an interfering effect on tests for low-load of HCV-RNA but not remarkably on those for high-load of HCV-RNA.
