中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2011年
1期
9-12
,共4页
于金玲%何开文%初文峰%朴贤美%乔国芬%吕延杰
于金玲%何開文%初文峰%樸賢美%喬國芬%呂延傑
우금령%하개문%초문봉%박현미%교국분%려연걸
三氧化二砷%白藜芦醇%NB4细胞%药物拮抗作用%细胞存活
三氧化二砷%白藜蘆醇%NB4細胞%藥物拮抗作用%細胞存活
삼양화이신%백려호순%NB4세포%약물길항작용%세포존활
Arsenic trioxide%Resveratrol%NB4 cells%Drug antagonism%Cell survival
目的 观察三氧化二砷(ATO)与白藜芦醇(Res)联用对人急性早幼粒细胞白血病细胞株NB4细胞存活率的影响,并探讨其作用机制.方法 采用不同剂量的ATO[0(对照)、0.1875、0.3750、0.7500、1.1250、1.5000、2.2500、3.0000、5.0000 μmol/L]、Res[0(对照)、1.5625、3.1250、6.2500、12.5000、18.7500、25.0000、37.5000、50.0000 μmol/L]孵育NB4细胞.应用四氮唑盐比色法(MTT)检测不同处理组的细胞存活率,计算ATO和Res对NB4细胞的半数抑制浓度(IC50).根据IC50,设置联合用药比例分别为1.5∶18、.5∶25、.5∶35,并根据药物联合指数计算公式,分别计算出联合用药在不同抑制效应(0.1、0.2、0.3、0.4、0.5、0.6、0.7、0.8、0.9)时的联合指数,判断ATO与Res在不同抑制效应时各自所需药物浓度之间的相互作用.应用DCFH-DA荧光探针检测对照组、ATO(1.5000 μmol/L)加药组、Res(25.0000 μmol/L)加药组、联合用药组(0.9000 μmol/L ATO+12.5000 μmol/L Res)NB4细胞活性氧(ROS)水平,每组各检测10份样品.结果 ①ATO(≥0.7500 μmol/L)加药组NB4细胞存活率明显低于对照组(P＜0.05),呈剂量依赖性的量效关系,IC50为(1.78±0.11)μmol/L.②Res(≥18.7500 μmol/L)加药组NB4细胞存活率明显低于对照组(P＜0.05),呈剂量依赖性的量效关系,IC50为(18.71±0.18)μmol/L.③ATO和Res联合应用对NB4细胞存活抑制效应呈现拮抗作用.④Res加药组NB4细胞内ROS水平(1670.55±13.97)明显低于对照组(2345.88±14.48,P＜0.05).ATO加药组NB4细胞内ROS水平(3092.42±94.84)明显高于对照组(P＜0.05).联合用药组NB4细胞内ROS水平(1860.27±15.99)明显低于ATO加药组(P＜0.05).结论 ATO、Res均可致NB4细胞存活率下降,而两者联合应用时表现为拮抗效应,ROS可能参与了这种拮抗作用.
目的 觀察三氧化二砷(ATO)與白藜蘆醇(Res)聯用對人急性早幼粒細胞白血病細胞株NB4細胞存活率的影響,併探討其作用機製.方法 採用不同劑量的ATO[0(對照)、0.1875、0.3750、0.7500、1.1250、1.5000、2.2500、3.0000、5.0000 μmol/L]、Res[0(對照)、1.5625、3.1250、6.2500、12.5000、18.7500、25.0000、37.5000、50.0000 μmol/L]孵育NB4細胞.應用四氮唑鹽比色法(MTT)檢測不同處理組的細胞存活率,計算ATO和Res對NB4細胞的半數抑製濃度(IC50).根據IC50,設置聯閤用藥比例分彆為1.5∶18、.5∶25、.5∶35,併根據藥物聯閤指數計算公式,分彆計算齣聯閤用藥在不同抑製效應(0.1、0.2、0.3、0.4、0.5、0.6、0.7、0.8、0.9)時的聯閤指數,判斷ATO與Res在不同抑製效應時各自所需藥物濃度之間的相互作用.應用DCFH-DA熒光探針檢測對照組、ATO(1.5000 μmol/L)加藥組、Res(25.0000 μmol/L)加藥組、聯閤用藥組(0.9000 μmol/L ATO+12.5000 μmol/L Res)NB4細胞活性氧(ROS)水平,每組各檢測10份樣品.結果 ①ATO(≥0.7500 μmol/L)加藥組NB4細胞存活率明顯低于對照組(P＜0.05),呈劑量依賴性的量效關繫,IC50為(1.78±0.11)μmol/L.②Res(≥18.7500 μmol/L)加藥組NB4細胞存活率明顯低于對照組(P＜0.05),呈劑量依賴性的量效關繫,IC50為(18.71±0.18)μmol/L.③ATO和Res聯閤應用對NB4細胞存活抑製效應呈現拮抗作用.④Res加藥組NB4細胞內ROS水平(1670.55±13.97)明顯低于對照組(2345.88±14.48,P＜0.05).ATO加藥組NB4細胞內ROS水平(3092.42±94.84)明顯高于對照組(P＜0.05).聯閤用藥組NB4細胞內ROS水平(1860.27±15.99)明顯低于ATO加藥組(P＜0.05).結論 ATO、Res均可緻NB4細胞存活率下降,而兩者聯閤應用時錶現為拮抗效應,ROS可能參與瞭這種拮抗作用.
목적 관찰삼양화이신(ATO)여백려호순(Res)련용대인급성조유립세포백혈병세포주NB4세포존활솔적영향,병탐토기작용궤제.방법 채용불동제량적ATO[0(대조)、0.1875、0.3750、0.7500、1.1250、1.5000、2.2500、3.0000、5.0000 μmol/L]、Res[0(대조)、1.5625、3.1250、6.2500、12.5000、18.7500、25.0000、37.5000、50.0000 μmol/L]부육NB4세포.응용사담서염비색법(MTT)검측불동처리조적세포존활솔,계산ATO화Res대NB4세포적반수억제농도(IC50).근거IC50,설치연합용약비례분별위1.5∶18、.5∶25、.5∶35,병근거약물연합지수계산공식,분별계산출연합용약재불동억제효응(0.1、0.2、0.3、0.4、0.5、0.6、0.7、0.8、0.9)시적연합지수,판단ATO여Res재불동억제효응시각자소수약물농도지간적상호작용.응용DCFH-DA형광탐침검측대조조、ATO(1.5000 μmol/L)가약조、Res(25.0000 μmol/L)가약조、연합용약조(0.9000 μmol/L ATO+12.5000 μmol/L Res)NB4세포활성양(ROS)수평,매조각검측10빈양품.결과 ①ATO(≥0.7500 μmol/L)가약조NB4세포존활솔명현저우대조조(P＜0.05),정제량의뢰성적량효관계,IC50위(1.78±0.11)μmol/L.②Res(≥18.7500 μmol/L)가약조NB4세포존활솔명현저우대조조(P＜0.05),정제량의뢰성적량효관계,IC50위(18.71±0.18)μmol/L.③ATO화Res연합응용대NB4세포존활억제효응정현길항작용.④Res가약조NB4세포내ROS수평(1670.55±13.97)명현저우대조조(2345.88±14.48,P＜0.05).ATO가약조NB4세포내ROS수평(3092.42±94.84)명현고우대조조(P＜0.05).연합용약조NB4세포내ROS수평(1860.27±15.99)명현저우ATO가약조(P＜0.05).결론 ATO、Res균가치NB4세포존활솔하강,이량자연합응용시표현위길항효응,ROS가능삼여료저충길항작용.
Objective To investigated the effects of combined arsenic trioxide(ATO) and resveratrol(Res)on the viability of NB4 human leukemia cells. Methods NB4 human leukemia cell was used in this experiment.Cells were cultured in ATO (0,0.1875,0.3750,0.7500, 1.1250, 1.5000,2.2500,3.0000,5.0000 μmol/L) and Res (0, 1.5625,3.1250,6.2500, 12.5000, 18.7500,25.0000,37.5000,50.0000 μmol/L). Cell viabilities were measured by MTT in different treatment groups. Half inhibitory concentration(IC50) was calculated. The ratio of concentration of ATO and Res 1.5∶ 18,1.5∶ 25,1.5∶ 35 was added to cells, and the combination index(CI) was calculated. The level of ROS in control, ATO( 1.5000 μmol/L), Res(25.0000 μmol/L) and ATO(0.9000 μmol/L) + Res( 12.5000μmol/L) groups was measured by chemiluminescence assay. Results ①ATO( ≥0.7500 μmol/L) reduced the viability of NB4 cells in a concentration-dependent manner(P ＜ 0.05 ), and IC50 was (1.78 ± 0.11 )μmol/L. ②)Res (≥18.7500 μ mol/L) dose-dependently decreased the viability of NB4 cells (P ＜ 0.05 ), and IC50 was ( 18.71 ±0.18)μ mol/L. ③Combination of ATO and Res showed an antagonistic effect on NB4 cells viability. ④The ROS in Res group( 1670.55 ± 13.97) was significantly lower than that in control group(2345.88 ± 14.48,P ＜ 0.05). The ROS in ATO group (3092.42 ± 94.84) was significantly higher than that in control group(P ＜ 0.05). The ROS in ATO + Res group (1860.27 ± 15.99) was significantly lower than that in ATO group(P ＜ 0.05). Conclusions NB4 cell survival rate can be decreased by ATO and Res. The combination of arsenic trioxide and Res presents an antagonistic effect on NB4 cell viability, in part by reducing intracellular ROS formation.
