CAJ | 학술논문

目的探讨神经细胞粘附分子(NCAM)及转录因子OCT-4在人隐睾中的病理表达及意义.方法 48例隐睾患儿,年龄6个月～13.1岁,平均3岁,单侧43例,双侧5例,共50个隐睾组织活检样本.免疫组化法检测生精小管和间质内NCAM、OCT-4的表达情况.结果 50个样本中,NCAM生精小管内阴性、睾丸间质细胞(Lc)阳性、OCT-4阴性的牛理规律表达率为38%(19/50).NCAM牛精小管、Lc中病理表达率为58%(29/50).NCAM生精小管内阳性病理表达率为30%(15/50),与年龄、睾丸位置无相关性(P>0.05).NCAM于Lc内阴性病理表达率为32%(16/50),与睾丸位置无相关性(P>0.05),但随年龄增加,阳性表达率增加(P<0.05).OCT-4阳性病理表达率为12%(6/50),与睾丸位置无相关性(P>0.05).结论 NCAM和OCT-4在隐睾中的病理性表达反映了生殖母细胞的迁移分化障碍或延迟,可以被作为预测隐睾生殖潜能和癌变趋势的可靠分子标志物.
목적 탐토신경세포점부분자(NCAM)급전록인자OCT-4재인은고중적병리표체급의의.방법 48례은고환인,년령6개월～13.1세,평균3세,단측43례,쌍측5례,공50개은고조직활검양본.면역조화법검측생정소관화간질내NCAM、OCT-4적표체정황.결과 50개양본중,NCAM생정소관내음성、고환간질세포(Lc)양성、OCT-4음성적우리규률표체솔위38%(19/50).NCAM우정소관、Lc중병리표체솔위58%(29/50).NCAM생정소관내양성병리표체솔위30%(15/50),여년령、고환위치무상관성(P>0.05).NCAM우Lc내음성병리표체솔위32%(16/50),여고환위치무상관성(P>0.05),단수년령증가,양성표체솔증가(P<0.05).OCT-4양성병리표체솔위12%(6/50),여고환위치무상관성(P>0.05).결론 NCAM화OCT-4재은고중적병이성표체반영료생식모세포적천이분화장애혹연지,가이피작위예측은고생식잠능화암변추세적가고분자표지물.
Objective To investigate the expressions of neural cell adhesion molecule (NCAM) and Octamer transcription factor-4 (OCT-4) in cryptorchid testes.Methods This study recruits 50 specimens from 48 children with cryptorchidism with a mean age of 3 years (range, 0.5-13.1 years).The specimens were collected by testicular biopsy during orchidopexy.Of the 48 patients, 43 had unilateral and 5 with bilateral cryptorchidism.The expressions of NCAM and (OCT-4 in seminiferous tubule or Leydig cells were detected by immunohistochemistry staining.Results The pathological expression, which was characterized by NCAM was positive in seminiferous tubule and Leydig cells, was 58%(29/50).Positive NCAM pathological expression in seminiferous tubule was 30% (15/50) with no relationship to the testicular locations or patients' age.Negative NCAM pathological expression in Leydig cells was 32% (16/50) with no relationship to the testicular locations.However, NCAM pathological expression in Leydig cells significantly increased with age (P<0.05).The OCT-4 was positive in seminiferous tubule in 6 of 50 patients (12 %), no significant difference was found between different tesicular locations (P>0.05).Conclusions The pathological expressions of NCAM and OCT-4 in cryptorchid testes indicate the migration and differentiation of gonocytes may be affected or delayed.NCAM and OCT-4 can be used as new diagnostic predictors for the infertility potential and carcinogenesis in cryptorchidism.