CAJ | 학술논문

目的观察小剂量瑞芬太尼(remifentanil,RF)对感染性休克幼兔体动脉血管环张力影响并探讨其机制.方法健康幼兔12只,分次制作36条幼兔离体体动脉血管环,随机分为6组:正常对照组、大肠杆菌脂多糖(lipopoly-saccharide,LPS)对照组、正常+RF组、LPS+ RF组、正常+N-硝基左旋精氨酸(L-NNA)+RF组和LPS+ L-NNA+ RF组,每组6个血管环.通过血管环张力测定,观察小剂量RF对感染性休克幼兔体动脉血管环反应性的影响,并观察预先以NO合成酶抑制剂L-NNA干预后的变化.结果正常组体动脉:与NS组比较,加入小剂量RF(4 μg/L)后各时间点(5 min、10 min、20 min)均引起体动脉明显的舒张反应,舒张幅度分别为(18.48±3.96)％、(23.63±4.42)％、(28.33±3.73)％(P＜0.05).预先以L-NNA干预后,上述舒张反应明显降低至(8.15±1.01)％、(13.08±1.46)％、(18.54±2.94)％(P＜0.05).LPS孵育后,小剂量RF(4 μg/L)对幼兔体动脉不具舒张作用,与加入NS组比较差异无统计学意义(P＞0.05),预先以L-NNA干预后,体动脉对该剂量RF张力变化差异无统计学意义(P＞0.05).结论小剂量RF对正常幼兔体动脉具有舒张作用,与NO产生有关；对LPS孵育的幼兔体动脉不具有舒张作用,对体动脉的张力调节与NO无关.
목적 관찰소제량서분태니(remifentanil,RF)대감염성휴극유토체동맥혈관배장력영향병탐토기궤제.방법건강유토12지,분차제작36조유토리체체동맥혈관배,수궤분위6조:정상대조조、대장간균지다당(lipopoly-saccharide,LPS)대조조、정상+RF조、LPS+ RF조、정상+N-초기좌선정안산(L-NNA)+RF조화LPS+ L-NNA+ RF조,매조6개혈관배.통과혈관배장력측정,관찰소제량RF대감염성휴극유토체동맥혈관배반응성적영향,병관찰예선이NO합성매억제제L-NNA간예후적변화.결과정상조체동맥:여NS조비교,가입소제량RF(4 μg/L)후각시간점(5 min、10 min、20 min)균인기체동맥명현적서장반응,서장폭도분별위(18.48±3.96)％、(23.63±4.42)％、(28.33±3.73)％(P＜0.05).예선이L-NNA간예후,상술서장반응명현강저지(8.15±1.01)％、(13.08±1.46)％、(18.54±2.94)％(P＜0.05).LPS부육후,소제량RF(4 μg/L)대유토체동맥불구서장작용,여가입NS조비교차이무통계학의의(P＞0.05),예선이L-NNA간예후,체동맥대해제량RF장력변화차이무통계학의의(P＞0.05).결론소제량RF대정상유토체동맥구유서장작용,여NO산생유관；대LPS부육적유토체동맥불구유서장작용,대체동맥적장력조절여NO무관.
Objective To investigate the effect and mechanism of low-dose remifentanil(RF)on systemic artery tension of baby rabbit with septic shock.Methods Thirty-six systemic artery strips were prepared from 12 baby rabbits and randomly divided into 6 groups:control group,lipopoly-saccharide(LPS)group,RF group,LPS + RF group,NG-nitro-L-arginine(L-NNA)pretreated group,LPS + L-NNA pretreated group.Each group had six rings(n =6).The effect of low-dose RF on the systemic artery tension of baby rabbit with septic shock was observed with isolated vascular ring technique.The change was also obversed after L-NNA pretreatment.Results In control group:the relaxant rates after low-dose RF at the time point of 5 min,10 min,20 min were(18.48±3.96)％,(23.63±4.42)％,(28.33±3.73)％(P＜0.05),compared with NS group.After pretreatment with L-NNA,RF-related relaxant rates of systemic arteries decreased significantly to(8.15 ± 1.01)％,(13.08 ± 1.46)％,(18.54 ±2.94)％(P ＜ 0.05).In LPS group,low dose RF(4 μg/L)did not bring out any response to systemic arteries(P ＞0.05).The tension was not affected with pretreatment of L-NNA(P ＞ 0.05).Conclusion These results suggest that low dose RF has relaxant effect on systemic arteries of baby rabbits,and NO may be involved.Low dose RF has no relaxant effect on LPS-pretreated systemic arteries of baby rabbits,and it has no association with NO.